| 货号 | 14132-100ug |
| 描述 | Isocitrate dehydrogenases (IDHs) are NAD+ and NADP+-dependent enzymes that catalyze the third step of the tricarboxylic acid cycle. IDHs catalyze oxidative decarboxylation of isocitrate producing α-ketoglutarate (α-KG) and carbon dioxide. IDH1 (cytosolic) and IDH2 (mitochondrial) are NADP+-dependent enzymes that catalyze reversible reactions. The IDH3 isoform, a NAD+-dependent multisubunit enzyme, is irreversible and allosterically regulated by a variety of positive (calcium, ADP, and citrate) and negative (ATP, NADH, and NADPH) effectors.1 IDH1 and IDH2 are mutated in >70% of lower grade gliomas.2 The most common IDH mutation, Arg132His, imparts new gain of function catalytic activity leading to the NADPH-dependent conversion of α-KG to 2-hydroxyglutarate.3,4 Astrocytes expressing IDH1 R132H mutant have been shown to produce markedly increased levels of the R-2-hydroxyglutarate enantiomer, leading to transformation of cells through the HIF prolyl 4-hydroxylase, EGLN.5 |
| 别名 | Isocitrate Dehydrogenase (NADP) Cytoplasmic; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Raimundo, N.,Baysal, B.E. and Shadel, G.S. Revisiting the TCA cycle: Signaling to tumor formation. Trends in Molecular Medicine 17(11), 641-649 (2011). 2.Turcan, S.,Rohle, D.,Goenka, A., et al. IDH1 mutation is sufficient to establish the glioma hypermethylator phenotype. Nature 483(7390), 479-483 (2012). 3.Reitman, Z.J. and Yan, H. Isocitrate dehydrogenase 1 and 2 mutations in cancer: Alterations at a crossroads of cellular metabolism. Journal of the National Cancer Institute 102(13), 932-941 (2010). 4.Dang, L.,White, D.W.,Gross, S., et al. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature 462(7274), 739-744 (2009). 5.Koivunen, P.,Lee, S.,Duncan, C.G., et al. Transformation by the (R)-enantiomer of 2-hydroxyglutarate linked to EGLN activation. Nature 483(7390), 484-488 (2012). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥95% estimated by SDS-PAGE |
| 稳定性 | ≥ 6 months |
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