| 货号 | 500830-96Well |
| 描述 | Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response.1,2,3,4,5,6 OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate tissue-specific effector functions in multiple mouse models of chronic inflammation, including allergic asthma, allergic rhinitis, and cutaneous hypersensitivity.1,2,3,4When using one of these models, it is often desirable to measure anti-OVA antibody levels in the plasma or serum to determine the effectiveness of the immunization, the activity of a drug, or the effect of a specific gene deletion. IgG1 is the predominant anti-OVA immunoglobulin isotype found in the serum or plasma of mice immunized with OVA/alum; the plasma concentration of OVA-specific IgG1 is typically 1,000-fold greater than that of OVA-specific IgE.1 Therefore, the measurement of anti-OVA IgG1 is a commonly used method of assessing the magnitude of this TH2 immune response.Cayman’s Anti-Ovalbumin IgG1 (mouse) ELISA Kit is an immunometric (i.e., “sandwich”) assay which can be used to measure anti-ovalbumin of the IgG1 isotype in mouse plasma and serum without prior sample purification. Affinity-purified anti-ovalbumin IgG1 isolated from the plasma of mice immunized with OVA/alum is used as the standard. The standard curve spans the range of 1.56-200 ng/ml, with an LLOQ of 1.56 ng/ml. |
| 别名 | Anti-OVA IgG1 (mouse) EIA Kit; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Hogan, S.P.,Mould, A.,Kikutani, H., et al. Aeroallergen-induced eosinophilic inflammation, lung damage, and airways hyperreactivity in mice can occur independently of IL-4 and allergen-specific immunoglobulins. Journal of Clinical Investigation 99(6), 1329-1339 (1997). 2.Kennedy, J.D.,Hatfield, C.A.,Fidler, S.F., et al. Phenotypic characterization of T lymphocytes emigrating into lung tissue and the airway lumen after antigen inhalation in sensitized mice. American Journal of Respiration, Cell, and Molecular Biology 12(6), 613-623 (1995). 3.Saito, H.,Howie, K.,Wattie, J., et al. Allergen-induced murine upper airway inflammation: Local and systemic changes in murine experimental allergic rhinitis. Immunology 104(2), 226-234 (2001). 4.Sawada, K.,Nagai, H.,Basaki, Y., et al. The expression of murine cutaneous late phase reaction requires both IgE antibodies and CD4 T cells. Clinical and Experimental Allergy 27(2), 225-231 (1996). 5.Pichavant, M.,Goya, S.,Hamelmann, E., et al. Animal models of airway sensitization. Current Protocols in Immunology 79, 15.18.11-15.18.19 (2007). 6.Kung, T.T.,Jones, H.,Adams, G.K., III, et al. Characterization of a murine model of allergic pulmonary inflammation. International Archives of Allergy and Immunology 105(1), 83-90 (1994). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | 4 |
| 稳定性 | ≥ 6 months |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |