O6-methylguanine-DNA Methyltransferase (human recombinant)
货号：
11176-100ug 基本售价：
4410.0 元 规格：
100 ug
产品信息
概述| 货号 | 11176-100ug |
| 描述 | O6-methylguanine-DNA Methyltransferase (MGMT) is a DNA repair enzyme responsible for demethylating O6-methylguanine (O6-mG).1 The methylation of guanine at O6 to form O6-mG allows base-pairing to thymine rather than cytosine during DNA replication. MGMT prevents mutations by transferring the methyl group from O6-mG to MGMT cysteine 145, restoring guanine. This transfer results in a covalent bond between MGMT cysteine 145 and the methyl group, and so MGMT is a single-turnover, "suicide" enzyme. The assay used to test MGMT is the demethylation of a synthesized, double-stranded, DNA oligonucleotide.2 Demethylation removes an O6-methyl group to expose a restriction site for the restriction endonuclease PvuII. Lanes A-E show 200 ng of methylated dsDNA treated with increasing amounts of MGMT prior to PvuII digestion. |
| 别名 | MGMT; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Mitra, S. MGMT: A personal perspective. DNA Repair (Amst) 6(8), 1064-1070 (2007).
2.Watts, G.S.,Pieper, R.O.,Costello, J.F., et al. Methylation of discrete regions of the O6-methylguanine DNA methyltransferase (MGMT) CpG island is associated with heterochromatinization of the MGMT transcription start site and silencing of the gene. Mol.Cell Biol. 17(9), 5612-5619 (1997).
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| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥70% estimated by SDS-PAGE |
| 稳定性 | ≥ 6 months |
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