| 货号 | 751-VE-025 |
| 别名 | vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRFVEGFL | 全称 | Vascular Endothelial Growth Factor B |
| 反应种属 | Human |
| 来源 | E. coli-derived Pro22-Arg188 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7423 (Human); 22339 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model. | |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its binding ability in a functional ELISA. Immobilized rrNeuropilin-1/Fc Chimera at 4 µg/mL (100 µL/well) can bind rhVEGF-B167 with a linear range of 0.3‑20 ng/mL. |
| 溶解方法 | Reconstitution in 4 mM HCl at 500 ug/mL. |
| 背景 | Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity (1, 2). Five mammalian members, including VEGF-A, -B, -C, -D and PlGF, have been identified. VEGF family members are disulfide-linked dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis and lymphangiogenesis. VEGF-B is expressed in most tissues, especially in heart, skeletal muscle and pancreas. In many tissues, VEGF-B is co-expressed and can heterodimerize with VEGF (3). By alternative splicing, two isoforms of mature VEGF-B containing 167 or 186 amino acid (aa) residues exist (3, 4). The two VEGF-B isoforms have identical amino-terminal cysteine-knot VEGF homology domains but the carboxyl end of VEGF-B167 differs from that of VEGF-B186 by the presence of a highly basic cysteine-rich heparin binding domain. Whereas VEGF-B186 is a secreted diffusible protein, VEGF-B167 is sequestered into the cell matrix after secretion. Both VEGF-B isoforms bind VEGF receptor 1 (VEGF R1), but not VEGF R2 or VEGF R3 (5). On endothelial cells, ligation of VEGF R1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1. VEGF-B167 and a proteolytically processed form of VEGF-B186(VEGF-B127) also bind neuropilin-1 (NP-1), a type I transmembrane receptor for semaphorins/collapsins, ligands involved in neuron guidance (6). Besides VEGF-B, NP-1 has been shown to bind PLGF-2, VEGF165 and VEGF R1 (6, 7). The many interactions of NP-1 with VEGF ligands and receptor suggests that NP-1 may function as a regulator of angiogenesis (7). |
| Accession # | AAA91463 |
| 运输条件 | Dry Ice |
| N-terminal Sequence Analysis | Pro22 |
| 预期分子量 | 19 (monomer) kDa |
| 存放说明 | -20℃ |
| 纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
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