| 货号 | 10944-100ug |
| 描述 | WD repeats are motifs of approximately 40 amino acids typically terminating in conserved tryptophan and aspartate residues. Several WD40 repeats combine to form the structural WD domain. Such an arrangement facilitates protein-protein interactions allowing the formation of multiprotein complexes.1 Human WDR5 contains seven WD 40 repeats and was originally identified to accelerate osteoblast differentiation.2 WDR5 has been demonstrated to bind histone H3 by recognizing the first three amino acids of the N-terminal tail.3 Binding of WDR5 to a conserved arginine-containing motif in MLL-1, the so-called WDR5 interaction (“Win”) motif, promotes the assembly and activity of the MLL core complex.4 Additional interactions have been demonstrated with other components of the human MLL core protein complex, which includes ASH2L and RbBP5.5,6 MLL1-5 protein complexes catalyze the di- and trimethylation of histone H3 at lysine 4 (H3K4me2/me3), leading to the maintenance of global H3K4 trimethylation.7 |
| 别名 | BIG3;BMP2-induced 3-kb Gene Protein;SWD3, Set1c WD40 repeat protein, homolog;WD-Repeat Protein 5; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Neer, E.J.,Schmidt, C.J.,Nambudripad, R., et al. The ancient regulatory-protein family of WD-repeat proteins. Nature 371(6495), 297-300 (1994). 2.Gori, G.,Divieti, P. and Demay, M.B. Cloning and characterization of a novel WD-40 repeat protein that dramatically accelerates osteoblastic differentiation. The Journal of Biological Chemisty 276(49), 46515-46522 (2001). 3.Couture, J.F.,Collazo, E. and Trievel, R.C. Molecular recognition of histone H3 by the WD40 protein WDR5. Nature Structural & Molecular Biology 13(8), 698-703 (2006). 4.Patel, A.,Vought, V.E.,Dharmarajan, V., et al. A conserved arginine-containing motif crucial for the assembly and enzymatic activity of the mixed lineage leukemia protein-1 core complex. The Journal of Biological Chemisty 283(47), 32162-32175 (2008). 5.Odho, Z.,Southall, S.M. and Wilson, J.R. Characterization of a novel WDR5-binding site that recruits RbBP5 through a conserved motif to enhance methylation of histone H3 lysine 4 by mixed lineage leukemia protein-1. The Journal of Biological Chemisty 285(43), 32967-32976 (2010). 6.Dou, Y.,Milne, T.A.,Ruthenburg, A.J., et al. Regulation of MLL1 H3K4 methyltransferase activity by its core components. Nature Structural & Molecular Biology 13(8), 713-719 (2006). 7.Wang, P.,Lin, C.,Smith, E.R., et al. Global analysis of H3K4 methylation defines MLL family member targets and points to a role for MLL1-mediated H3K4 methylation in the regulation of transcriptional initiation by RNA polymerase II. Molecular and Cellular Biology 29(22), 6074-6085 (2009). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥95% estimated by SDS-PAGE |
| 稳定性 | ≥ 1 year |
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