| 货号 | 19546-50ug |
| 描述 | Pertussis toxin (islet-activating protein) is a toxin, first isolated from B. pertussis, that is used to study G protein-coupled receptor signaling in cells and experimental autoimmune encephalomyelitis (EAE) in animals. Pertussis toxin catalyzes the transfer of the ADP-ribose moiety of NAD to the α subunits of heterotrimeric Gi/o proteins, resulting in the receptors being uncoupled from Gi/oproteins.1,2 Pertussis toxin is also used as an adjuvant, given with specific antigens, to immunize animals to produce EAE, an animal model of multiple sclerosis.3,4 Pertussis toxin was first described as an islet-activating protein because it caused a sustained potentiation of the secretory response of pancreatic islet cells to various stimuli that stimulate Gi-linked α-adrenergic receptors.5,6 |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Kaslow, H.R. and Burns, D.L. Pertussis toxin and target eukaryotic cells: Binding, entry, and activation. FEBS Journal 6(9), 2684-2690 (1992). 2.Ui, M. Islet-activating protein, pertussis toxin: A probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclase. Trends in Pharamacological Sciences 5, 277-279 (1984). 3.Hofstetter, H.H.,Shive, C.L. and Forsthuber, T.G. Pertussis toxin modulates the immune response to neuroantigens injected in incomplete Freund’s adjuvant: Induction of Th1 cells and experimental autoimmune encephalomyelitis in the presence of high frequencies of Th2 cells. Journal of Immunology 169(1), 117-125 (2002). 4.Ronchi, F.,Basso, C.,Preite, S., et al. Experimental priming of encephalitogenic Th1/Th17 cells requires pertussis toxin-driven IL-1β production by myeloid cells. Nat.Commun. 7:11541, (2016). 5.Heyworth, C.M.,Grey, A.M.,Wilson, S.R., et al. The action of islet activating protein (pertussis toxin) on insulin’s ability to inhibit adenylate cyclase and activate cyclic AMP phosphodiesterases in hepatocytes. Biochemistry Journal 235(1), 145-149 (1986). 6.Katada, T. and Michio, U. Slow interaction of islet-activating protein with pancreatic islets during primary culture to cause reversal of α-adrenergic inhibition of insulin secretion. The Journal of Biological Chemisty 255(20), 9580-9588 (1980). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | 2-8 |
| 纯度 | ≥95% (estimated by SDS-PAGE) |
| CAS号 | 70323-44-3 |
| 稳定性 | ≥ 2 years |
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