| 货号 | 11235-100ug |
| 描述 | The heterochromatin protein 1 (HP1) family, which consists of three isoforms HP1α (CBX5), HP1β (CBX1) and HP1γ (CBX3) are chromatin-associated proteins involved in gene regulation and heterochromatin formation.1 CBX proteins have an N-terminal chromodomain, a C-terminal chromoshadow domain, and a hinge domain which connects the two.2 Extensive post-translational modifications have been observed and mapped on the CBX proteins.3 The chromodomain of CBX5 has been shown to recognize di- and tri-methylated lysine 9 on histone H3 (H3K9me2 and H3K9me3), with a preference for H3K9me3. Binding of CBX5 to methylated histones leads to gene silencing and heterochromatin formation.1,4 H3K9 trimethylation by the methyltransferase SETDB1 creates a binding site for recruitment of CBX5, leading to chromatin condensation and gene silencing.5 The chromoshadow domain facilitates protein-protein interactions like homodimerization and recruitment of nuclear proteins involved in transcriptional regulation.6 The chromoshadow domain is also responsible for recruitment to sites of DNA damage, where CBX5 helps to reorganize chromatin as part of the DNA damage response system.7 |
| 别名 | Antigen p25;Chromobox Protein Homolog 5;Heterochromatin Protein 1-α;HP1-α; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Lachner, M.,OCarroll, D.,Rea, S., et al. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins. Nature 410, 116-120 (2001). 2.Kwon, S.H. and Workman, J.L. The changing faces of HP1: From heterochromatin formation and gene silencing to euchromatic gene expression. BioEssays 33(4), 280-289 (2011). 3.LeRoy, G.,Weston, J.T.,Zee, B.M., et al. Heterochromatin protein 1 is extensively decorated with histone code-like post-translational modifications. Molecular & Cellular Proteomics 8(11), 2432-2442 (2009). 4.Kaustov, L.,Ouyang, H.,Amaya, M., et al. Recognition and specificity determinants of the human Cbx chromodomains. The Journal of Biological Chemisty 286(1), 521-529 (2011). 5.Verschure, P.J.,van der Kraan, I.,de Leeuw, W., et al. In vivo HP1 targeting causes large-scale chromatin condensation and enhanced histone lysine methylation. Molecular and Cellular Biology 25(11), 4552-4564 (2005). 6.Richart, A.N.,Brunner, C.I.W.,Stott, K., et al. Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 α (HP1α) to histone H3. The Journal of Biological Chemisty 287, 18730-18737 (2012). 7.Luijsterburg, M.S.,Dinant, C.,Lans, H., et al. Heterochromatin protein 1 is recruited to various types of DNA damage. Journal of Cell Biology 185(4), 577-586 (2009). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥95% |
| 稳定性 | ≥ 6 months |
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