| 货号 | 5610/10 |
| 别名 | (5Z)-5-[(3,5-Difluoro-4-hydroxyphen |
| 供应商 | Tocris |
| 生物活性 | Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 纯度 | >98 % |
| 计算分子量 | 320.21 |
| 分子式 | C13H9F5N2 |
| 可溶性/溶解性 | Soluble to 100 mM in DMSO and to 50 mM in ethanol |
| 参考文献 | Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415. PMID: 26877022. Filonovet al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649. PMID: 26000751. Songet al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198. PMID: 24393009. |