Nitrate/Nitrite Fluorometric Assay Kit
货号：
780051-192Well 基本售价：
3388.0 元 规格：
2 x 96 wells
产品信息
概述| 货号 | 780051-192Well |
| 描述 | Nitric Oxide (NO) is produced in trace quantities by neurons, endothelial cells, platelets, and neutrophils in response to homeostatic stimuli. This NO is scavenged rapidly (t½ = 4 s) and acts in a paracrine fashion to transduce cellular signals. NO interacts with the HEME prosthetic group of guanylate cyclase, activating the enzyme and leading to increased cGMP levels. Other cells (macrophages, fibroblasts, and hepatocytes) also produce NO in micromolar concentrations in response to inflammatory or mitogenic stimuli. These very high levels of NO lead to the formation of peroxynitrite, destruction of iron-sulphur clusters, thiol nitrosation, and nitration of protein tyrosine residues. Thus, the amount of NO produced in different biological systems can vary over several orders of magnitude and its subsequent chemical reactivity is diverse. The final products of NO in vivo are nitrite (NO2-) and nitrate (NO3-). Relative proportions of NO2- and NO3- within a sample are variable and cannot be predicted with certainty. Therefore, the best index of total NO production is the sum of both of these products. Cayman Chemical’s Nitrate/Nitrite Fluorometric Assay Kit provides a convenient method for the quantitation of low levels of NO2- and NO3- in biological samples (particularly tissue culture medium). Previously undetectable concentrations of nitrate/nitrite in a variety of samples can be accurately quantitated using this kit as it extends the lower limit of detection by 20-fold over our popular colorimetric version. The minimum detectable quantity of NO2/NO3 is ~50 nM. |
| 别名 | Nitric Oxide Metabolite Detection Kit; |
性能| 供应商 | Cayman |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
