| 货号 | 703302-96Well |
| 描述 | Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble.1 The glutathione conjugates are metabolized further to mercapturic acid and then excreted.1 Based on their sequence homology, substrate specificity, and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes.2,3,4,5 These classes are comprised of cytosolic enzymes and the fifth form is microsomal.2,3,4,5 The Cayman Chemical Glutathione S-Transferase Assay Kit measures total GST activity (cytosolic and microsomal) by measuring the conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione.6 The conjugation is accompanied by an increase in absorbance at 340 nm. The rate of increase is directly proportional to the GST activity in the sample. The Cayman GST Assay Kit can be used to measure GST activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Cytosolic and microsomal GST activity can also be assayed separately. |
| 别名 | GST Assay Kit; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Boyland, E., and Chasseaud, L.F. The role of glutathione and glutathione S-transferases in mercapturic acid biosynthesis. Advances in Enzymology 32, 173-219 (1969). 2.Mannervik, B. The isoenzymes of glutathione transferase. Advances in Enzymology and Related Areas of Molecular Biology 57, 357-417 (1985). 3.Mannervik, B.,Ålin, P.,Guthenberg, C., et al. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: Correlation between structural data and enzymatic properties. Proceedings of the National Academy of Sciences of the United States of America 82, 7202-7206 (1985). 4.Morgenstern, R.,DePierre, J.W., and Ernster, L. Activation of microsomal glutathione S-transferase activity by sulfhydryl reagents. Biochemical and Biophysical Research Communications 87, 657-663 (1979). 5.Jakoby, W.B. The glutathione S-transferases: A group of multifunctional detoxification proteins. Advances in Enzymology and Related Areas of Molecular Biology 46, 383-414 (1978). 6.Habig, W.H.,Pabst, M.J., and Jakoby, W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. The Journal of Biological Chemisty 249, 7130-7139 (1974). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 6 months |
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