PAF Acetylhydrolase Assay Kit
货号：
760901-96Well 基本售价：
2926.0 元 规格：
96 wells
产品信息
概述| 货号 | 760901-96Well |
| 描述 | Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.1 The Cayman Chemical PAF acetylhydrolase assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH.2 Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5-dithio-bis(2-nitrobenzoic acid) (DTNB; Ellman’s reagent). The dynamic range of the assay is only limited by the accuracy of the absorbance measurement. Most plate readers are linear to an absorbance of 1.2. The detection range of the assay is from 0.02 to 0.2 µmol/min/ml of PAF-AH activity which is equivalent to an absorbance increase of 0.01 to 0.1 per minute. Each kit contains assay buffer, DTNB, 2-thio PAF (substrate), human PAF-AH standard, a 96 well plate, and complete instructions. |
| 别名 | Lp-PLA2;PAF-AH; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Stafforini, D.M.,Prescott, S.M. and McIntyre, T.M. Human plasma platelet-activating factor acetylhydrolase. The Journal of Biological Chemisty 262, 4223-4230 (1987).
2.Aarsman, A.J.,Neys, F.W. and van den Bosch, H. Catabolism of platelet-activating factor and its acyl analog. Differentiation of the activities of lysophospholipase and platelet-activating-factor acetylhydrolase. European Journal of Biochemistry 200, 187-193 (1991).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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