| 货号 | 9995S |
| 供应商 | CST |
| 背景 | Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7). |
| 参考文献 | Liu, C. and Hermann, T.E. (1978) J Biol Chem 253, 5892-4. Kauffman, R.F. et al. (1980) J Biol Chem 255, 2735-9. Morgan, A.J. and Jacob, R. (1994) Biochem J 300 ( Pt 3), 665-72. Lobo, F.M. et al. (1999) J Immunol 162, 2057-63. Chatila, T. et al. (1989) J Immunol 143, 1283-9. Aagaard-Tillery, K.M. and Jelinek, D.F. (1995) J Immunol 155, 3297-307. Lin, M.I. et al. (2003) J Biol Chem 278, 44719-26. |
Structure of Ionomycin Ionomycin的结构 | |
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Ionomycin (1 μM, 1 hr; right), using NFAT1 (D43B1) XP® Rabbit mAb #5861 (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
