| 货号 | 8177S |
| 供应商 | CST |
| 背景 | Novel, potent, and selective JNK-1,-2, and -3 inhibitor, SP600125 is an ATP-competetive inhibitor effective on a range of kinases and enzymes. In cells, SP600125 caused a dose-dependent inhibition of the phosphorylation of c-Jun, the expression of inflammatory genes IL-2, COX-2, TNF-α, IFN-γ, and blocked the activation and differentiation of primary human CD4 cell cultures (1). SP600125 has also demostrated inhibitory effects on tumor cell proliferation, endothelial cell migration, and tumor growth as well as blocking tumor and endothelial cells in the G2 phase of the cell cycle (2). |
| 存放说明 | -20C |
| 参考文献 | 1 . Bennett, B.L. et al. (2001) Proc Natl Acad Sci U S A 98, 13681-6. 2 . Ennis, B.W. et al. (2005) J Pharmacol Exp Ther 313, 325-32. |
Chemical structure of SP600125.SP600125的化学结构。 | |
Western blot analysis of extracts from 293T cells, untreated or treated with anisomycin (25 μg/ml) for the indicated times either with or without SP600125 pre-treatment (50 μM, 40 min), using Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053 (upper) or JunB (C37F9) Rabbit mAb #3753 (lower).Western blot分析293T细胞提取物，未处理或先用SP600125(50 μM, 40分钟)进行预处理或不预处理，再用茴香霉素(25 μg/ml)按照指示处理，使用的抗体是：Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053（上图）、JunB (C37F9) Rabbit mAb #3753（下图）。 | |
Western blot analysis of extracts from 293T cells, untreated or treated with anisomycin (25 μg/ml, 15 min) either with or without SP600125 pre-treatment (50 μM, 40 min), using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270 (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower).Western blot分析293T细胞提取物，未处理或先用SP600125(50 μM, 40分钟)进行预处理或不预处理，再用茴香霉素(25 μg/ml，15分钟)处理，使用的抗体是：Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270（上图）、c-Jun (60A8) Rabbit mAb #9165（下图）。 |
