| 货号 | 5633S |
| 使用方法 | SB203580 (p38 Inhibitor) is supplied as a lyophilized white powder. For 10 mM stock, resuspend 5 mg of the inhibitor in 1.32 ml DMSO. Aliquot and freeze at -20ºC to avoid multiple freeze/thaw cycles which can degrade the inhibitor. For experiments with cultured cells, we recommend pre-treating the cells with SB203580 at 10 µM for one to two hours prior to stimulation. This product is for in vitro research use only and is not intended for use in humans or animals. SB203580 (p38抑制剂)以冻干白色粉末状供应。10 mM储存时，5 mg抑制子重悬于1.32 ml DMSO中。分装并与于20ºC保存，避免冷冻/解冻循环。因为这样会损害抑制子。对于试验中的培养后细胞，我们建议在刺激之前，先将细胞用SB203580 10 µM处理1-2h。该产品只用于体内研究，不能用于人类和动物体中。 |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 参考文献 | Rouse, J. et al. (1994) Cell 78, 1027-37. Han, J. et al. (1994) Science 265, 808-11. Lee, J.C. et al. (1994) Nature 372, 739-46. Freshney, N.W. et al. (1994) Cell 78, 1039-49. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31. |
Molecular weight: 377.43 | |
Molecular formula: C21H16FN3OS | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or sorbitol-treated for 30 minutes with or without SB203580 pre-treatment (1 hr.), using Phospho-MAPKAPK-2 (Thr222) Antibody #3044 (upper) or MAPKAPK-2 Antibody #3042 (lower).western blot方法检测细胞提取物：未处理和sorbitol 30min处理的NIH/3T3细胞，用和未用SB203580提前处理1h，使用的抗体是Phospho-MAPKAPK-2 (Thr222) Antibody #3044 (上图)和MAPKAPK-2 Antibody #3042(下图)。 |
