SB202190【科瑞奥博】

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SB202190

货号： 8158S 基本售价： 2596.0 元规格： 5mg

产品信息

概述
货号	8158S

性能
供应商	CST
背景	SB202190, a pyridinyl imidazole, inhibits p38 MAP kinase activity through competition with ATP (1), and has been shown to induce apoptosis through activation of cysteine protease (CPP32)-like caspases (2). SB202190 blocks both lipopolysaccharide (LPS)-induced gene expression and nitric oxide (NO)-induced stabilization of interleukin (IL)-8 mRNA in monocytes (3,4). Pre-treatment of cells with SB202190 has been shown to inhibit phosphorylation of p38 MAPK despite exposure to anisomycin, a known inducer of the MAPK pathway (5). Of note, the mechanism of inhibition of SB202190 is unlike the one seen with SB203580, which inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (6).
存放说明	-20C
参考文献	1 . Young, P.R. et al. (1997) J Biol Chem 272, 12116-21. 2 . Nemoto, S. et al. (1998) J Biol Chem 273, 16415-20. 3 . Manthey, C.L. et al. (1998) J Leukoc Biol 64, 409-17. 4 . Ma, P. et al. (2004) J Leukoc Biol 76, 278-87. 5 . Geiger, P.C. et al. (2005) Am J Physiol Endocrinol Metab 288, E782-8. 6 . Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

参考图片

Chemical Structure of SB202190SB202190的化学结构。

Western blot analysis of extracts from Jurkat cells, serum-starved overnight and pretreated with the indicated amounts of SB202190 for 1 hr, and subsequently treated with anisomycin (25 ng/ml, 30 min), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).Western blot分析Jurkat细胞提取物，血清饥饿过夜并用推荐的SB202190的量预处理1小时，随后用茴香霉素(25 ng/ml, 30分钟)处理，使用的抗体是：Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511（上图）、p38 MAPK Antibody #9212（下图）。

Western blot analysis of extracts from Jurkat cells, serum-starved overnight and pretreated with SB202190 (10 µM, 1 hr), and untreated or treated with anisomycin (25 ng/ml, 30 min), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316 (upper) or MAPKAPK-2 Antibody #3042 (lower). MAPKAPK-2 is a specific downstream substrate of p38 MAPK.Western blot分析Jurkat细胞提取物，血清饥饿过夜并用SB202190 (10 µM, 1小时)预处理，用茴香霉素(25 ng/ml, 30分钟)处理或未处理，使用的抗体是：Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316（上图）、MAPKAPK-2 Antibody #3042（下图）。MAPKAPK-2是p38 MAPK的特异的下游底物。