| 货号 | 14774S |
| 反应种属 | All |
| 应用 | W |
| 使用方法 | Chloroquine is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 150 mg in 5.82 ml sterile dH2O. First add 1 ml dH2O to the tube containing the chemical, vortex, and dispense into a new, larger tube. Repeat this action two or three more times to transfer any residual material. Add additional dH2O to the new tube to bring the volume up to 5.82 ml. Filter sterilize into sterile tube. Utilize a syringe and 0.2 μm syringe filter to minimize sample loss. |
| 供应商 | CST |
| 背景 | Chloroquine (CQ) is a lysosomotropic agent with an extensive range of biological effects (1). Historically known for its anti-malarial activity, chloroquine is a widely used biological research tool for studying autophagy inhibition. Research studies demonstrate that chloroquine accumulates in acidic lysosomes and increases the lysosomal pH. This inhibits lysosomal hydrolases and prevents autophagosomal fusion and degradation, which can result in apoptotic or necrotic cell death (1-4). Inhibition of chloroquine-induced apoptosis with the V-ATPase inhibitor bafilomycin A1 has been observed in several cell types (4). Chloroquine also enhances the anti-neoplastic effects of the histone deacetylase inhibitor vorinostat (SAHA) (5). |
| 存放说明 | RT |
| 纯度 | >98% |
| 计算分子量 | 515.9 g/mol |
| 参考文献 | 1 . Liang, X. et al. (2012) Cancer Res 72, 2791-801. 2 . Liang, X. et al. (2014) Cell Biosci 4, 10. 3 . Kung, C.P. et al. (2011) Crit Rev Eukaryot Gene Expr 21, 71-100. 4 . Shacka, J.J. et al. (2006) Mol Pharmacol 69, 1125-36. 5 . Carew, J.S. et al. (2007) Blood 110, 313-22. |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated (-) or treated with Chloroquine (50 μM, overnight; +), using LC3A/B (D3U4C) XP® Rabbit mAb #12741. | |
Chemical structure of chloroquine. | |
Confocal immunofluorescent analysis of HeLa (upper) and C2C12 (lower) cells, treated with Chloroquine (50 μM, overnight; left), nutrient-starved with EBSS (3 hr; middle), or untreated (right), using LC3A/B (D3U4C) XP® Rabbit mAb #12741 (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). |
