| 货号 | 9493S |
| 供应商 | CST |
| 背景 | The staurosporine analog, PKC412, inhibits a variety of serine/threonine and tyrosine kinases including PKC (IC50 = 50 nM), cyclic AMP-dependent protein kinase (IC50 = 2.4 µM), S6 kinase (IC50 = 5.0 µM), and EGFR (epidermal growth factor receptor) tyrosine kinase activity (IC50 = 3.0 µM) (1). PKC412 has also demonstrated stong inhibitory effects on FLT3, which causes G1 cell cycle arrest and apoptosis (2). |
| 存放说明 | -20C |
| 参考文献 | 1 . Meyer, T. et al. (1989) Int J Cancer 43, 851-6. 2 . Bali, P. et al. (2004) Clin Cancer Res 10, 4991-7. |
Chemical structure of PKC412. PKC412的化学结构。 | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with Calyculin A #9902 (50 nM, 20 min) with or without pre-treatment with PKC412 (24 hr) for the indicated concentrations, using Phospho-(Ser) PKC Substrate Antibody #2261 (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).Western blot分析Jurkat细胞提取物，未处理或先用PKC412 (24小时)根据设计好的浓度进行预处理或不预处理，再用Calyculin A #9902 (50 nM, 20分钟)处理，使用的抗体是：Phospho-(Ser) PKC Substrate Antibody #2261（上图）、β-Actin (13E5) Rabbit mAb #4970（下图）。 | |
Western blot analysis of extracts from SEM cells, untreated (-) or treated with PKC412 (1 μM, 24 hr; +), using Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464 (upper) and FLT3 (8F2) Rabbit mAb #3462 (lower).Western blot分析SEM细胞提取物，未处理(-)或用PKC412 (1 μM, 24小时; +)处理，使用的抗体是：Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464（上图）、FLT3 (8F2) Rabbit mAb #3462（下图）。 |
