| 货号 | 12060S |
| 供应商 | CST |
| 背景 | Gö6976 is an ATP-competitive PKC inhibitor specific for Ca2+-dependent PKCα (IC50 = 2.3 nM) and PKCβ1 (IC50 = 6.2 nM) isozymes (1). It is also a potent inhibitor of PKD (IC50 = 20 nM) (2). Researchers have demonstrated that Gö6976 blocks JNK activation (3-5) and inhibits PKCα-mediated, TPA-stimulated phosphorylation of CREB at Ser133 (6,7). Gö6976 is also an effective inhibitor of the tyrosine kinases Jak2 and FLT3 (8), as well as TrkA and TrkB (9). |
| 存放说明 | -20C |
| 参考文献 | 1 . Martiny-Baron, G. et al. (1993) J Biol Chem 268, 9194-7. 2 . Gschwendt, M. et al. (1996) FEBS Lett 392, 77-80. 3 . López-Bergami, P. et al. (2005) Mol Cell 19, 309-20. 4 . Wen, J. et al. (2011) J Neuroinflammation 8, 38. 5 . Lemonnier, J. et al. (2004) J Biol Chem 279, 259-64. 6 . Chung, Y.W. et al. (2011) J Biol Chem 286, 29681-90. 7 . Paruchuri, S. and Sjölander, A. (2003) J Biol Chem 278, 45577-85. 8 . Grandage, V.L. et al. (2006) Br J Haematol 135, 303-16. 9 . Behrens, M.M. et al. (1999) J Neurochem 72, 919-24. |
Chemical Structure of Gö6976.Gö6976的化学结构。 | |
Western blot analysis of extracts from Jurkat cells, serum-starved overnight and untreated or treated with TPA #4174 (100 nM, 15 min) either with or without Gö6976 pretreatment (1 hr) at the indicated concentrations, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (upper) or SAPK/JNK (56G8) Rabbit mAb #9258 (lower).Western blot分析Jurkat细胞提取物，血清饥饿过夜，未处理或者先用Gö6976 (1小时)根据设计好的不同浓度进行预处理或不预处理，再用TPA #4174 (100 nM, 15分钟)处理，使用的抗体是：Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668（上图）、SAPK/JNK (56G8) Rabbit mAb #9258（下图）。 |
