| 货号 | 3828S |
| 供应商 | CST |
| 背景 | Forskolin, a naturally occurring diterpene from the Indian plant, Coleus forskohlii, activates adenylate cyclase, and thus increases the intracellular cAMP concentration (1). The second messenger cAMP activates cAMP-dependent protein kinase (PKA or cAPK) and controls many cellular mechanisms such as gene transcription, ion transport and protein phosphorylation (2). |
| 存放说明 | -20C |
| 参考文献 | Laurenza, A. et al. (1989) Trends Pharmacol Sci 10, 442-7. Montminy, M. (1997) Annu Rev Biochem 66, 807-22. |
Western blot analysis of extracts from SH-SY5Y cells, forskolin-treated (30 µM for 20 min.) or untreated, using Phospho-CREB (Ser133) Antibody #9191 (upper) or CREB Antibody #9192 (lower).对SH-SY5Y细胞抽提液，30 uM forskolin处理20分钟或未处理，使用Phospho-CREB (Ser133)抗体#9191（上图）或CREB抗体#9192（下图）进行Western blot分析。 |
