| 货号 | 9676S |
| 反应种属 | All Species Expected, |
| 应用 | IF-IC |
| 供应商 | CST |
| 背景 | Leptomycin B (LMB), originally discovered and utilized as a potent anti-fungal antibiotic from Streptomyces sp., has more recently been identified to inhibit nuclear export of proteins and RNA containing a Nuclear Export Sequence (NES) (1). The mechanism behind LMBs potent inhibition is achieved by specifically binding to chromosomal region maintenance (CRM)/exportin 1 protein; CRM1 binds to ribonuclear proteins containing the NES (1,2). LMB has also been reported to inhibit the degredation and subsequently lead to accumulation of p53 within the nucleus (3) and has demontrated specific anti-tumor properties, although toxic, at high doses (1-3). |
| 存放说明 | -20C |
| 参考文献 | 1 . Jang, B.C. et al. (2003) J Biol Chem 278, 2773-6. 2 . Mutka, S.C. et al. (2009) Cancer Res 69, 510-7. 3 . Hietanen, S. et al. (2000) Proc Natl Acad Sci U S A 97, 8501-6. |
Chemical structure of Leptomycin B来普霉素B的化学结构。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with LMB (200 nM, 18 hr ), using p53 (7F5) Rabbit mAb #2527 and β-Actin (13E5) Rabbit mAb #4970.Western blot分析HeLa细胞提取物，未处理(-)或用LMB (200 nM, 18小时，+)处理，使用的抗体是：p53 (7F5) Rabbit mAb #2527、β-Actin (13E5) Rabbit mAb #4970。 | |
Confocal immunofluorescent analysis of IGROV-1 cells, treated with LY294002 #9901 (50 μM, 3hr; left), insulin (100 ng/mL, 30 min; middle), or Leptomycin B followed by insulin (20 nM, 3hr and 100 ng/mL, 30 min, respectively; right), using FoxO1 (C29H4) Rabbit mAb #2880 (green). Actin filaments were labeled with DY-554 phalloidin (red).共聚焦免疫荧光分析IGROV-1细胞，分别用LY294002 #9901 (50 μM, 3小时;左图)、胰岛素 (100 ng/mL, 30分钟;中间)、来普霉素B和胰岛素(20 nM, 3小时和100 ng/mL, 30分钟, 右图)处理，使用的抗体是：FoxO1 (C29H4) Rabbit mAb #2880（绿色）。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。 |
