| 货号 | 1231/5 |
| 别名 | 1,6,7,8,9,11aβ,12,13,14,14αa-Decahydr |
| 供应商 | Tocris |
| 生物活性 | Reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor to the Golgi apparatus and inhibits GDP-GTP exchange. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 纯度 | >98 % |
| 计算分子量 | 280.36 |
| 分子式 | C16H24O4 |
| 可溶性/溶解性 | Soluble to 50 mM in DMSO and to 10 mM in ethanol |
| CAS号 | 20350-15-6 |
| 参考文献 | Yuet al (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16 142. PMID: 25658371. Dinget al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702. PMID: 17135238. Morinagaet al (1996) Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain. Proc.Natl.Acad.Sci.U.S.A. 93 12856. PMID: 8917509. Tsaiet al (1994) Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain. Proc.Natl.Acad.Sci.U.S.A. 91 3063. PMID: 8159707. Ktistakiset al (1992) Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 356 344. PMID: 1549178. |