| 货号 | 10990-10ug |
| 描述 | Glucokinase (GCK) is an enzyme that catalyses the ATP-dependent phosphorylation of glucose to produce glucose-6-phosphate. This is the first and rate-limiting step in the glycolytic pathway. GCK was initially described in liver but is present in other tissues as well, such as pancreatic B cells, neurons, the pituitary, and endocrine cells of the gut.1 Unlike other members of the hexokinase family (human hexokinases I-III), GCK has a high S0.5 value for glucose, the concentration giving half maximal activity. The S0.5 value of GCK for glucose is 5-8 mM, which is near the normal fasting blood glucose levels of 3.9-5.5 mM. In contrast, hexokinases I-III have S0.5 values for glucose in the range of 20-130 µM.2 The distribution of GCK-expressing cells and its sensitivity to glucose is believed to play a central role in maintaining glucose homeostasis. Mutations of the glucokinase gene are associated with non-insulin-dependent (type 2) diabetes mellitus (NIDDM).3 The kinetic defects associated with glucokinase mutations include reduced catalytic activity, increased KM for glucose, and/or increased KM for ATP.4 GCK activity in hepatocytes is responsible for regulating glycogen synthesis in response to changes in the ambient glucose concentrations.5 |
| 别名 | ATP:D-Hexose 6-Phosphotransferase GCK;Hexokinase D;Human Hexokinase IV isoform 2; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Massa, M.L.,Gagliardino, J.J. and Francini, F. Liver glucokinase: An overview on the regulatory mechanisms of its activity. Life 63(1), 1-6 (2011). 2.Mahalingam, B.,Cuesta-Munoz, A.,Davis, E.A., et al. Structural model of human glucokinase in complex with glucose and ATP. Diabetes 48, 1698-1705 (1999). 3.Tahrani, A.A.,Bailey, C.J.,Del Prato, S., et al. Management of type 2 diabetes: New and future developements in treatment. New Horizons 6736(11), 60207-60209 (2011). 4.Gidh-Jain, M.,Takeda, J.,Xu, L.Z., et al. Glucokinase mutations associated with non-insulin-dependent (type 2) diabetes mellitus have decreased enzymatic activity: Implications for structure/function relationships. Proceedings of the National Academy of Sciences of the United States of America 90, 1932-1936 (1993). 5.Matschinsky, F.M. Regulation of pancreatic ß-cell glucokinase. Diabetes 51(Suppl. 3), S394-S404 (2002). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 稳定性 | ≥ 6 months |
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