| 货号 | 89542-200ug |
| 描述 | This product can be used as a positive control for immunoblotting experiments using Cayman Chemical’s Nitrotyrosine Polyclonal Antibody (Catalog No. 189540) and Nitrotyrosine Monoclonal Antibody (Catalog No. 189542). Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide (6.7 x 109 M−1sec−1) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo.1,2 Nitrotyrosine has been shown to be present in proteins from a variety of clinical conditions including atherosclerotic lesions of human coronary arteries, postischemic heart, and placenta during preeclampsia.3,4,5 Increased nitration of proteins in motor neurons has been identified in patients with amyotrophic lateral sclerosis and may be due to mutations in superoxide dismutase.2,6,7,8 |
| 别名 | NT-BSA;Peroxynitrite-treated BSA; |
| 应用 | WB: 50-100 ng is recommended for use as a positive control |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Beckman, J.S.,Chen, J.,Ischiropoulos, H., et al. Oxidative chemistry of peroxynitrite. Methods in Enzymology 233, 229-240 (1994). 2.Beckman, J.S., and Koppenol, W.H. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly. American Journal of Physiology 271, C1424-C1437 (1996). 3.Beckman, J.S.,Ye, Y.Z.,Anderson, P.G., et al. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biological Chemistry of Hoppe-Seyler 375, 81-88 (1994). 4.Wang, P., and Zweier, J.L. Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite-mediated reperfusion injury. The Journal of Biological Chemisty 271, 29223-29230 (1996). 5.Myatt, L.,Rosenfield, R.B.,Eis, A.L.W., et al. Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action. Hypertension 28, 488-493 (1996). 6.Abe, K.,Pan, L.H.,Watanabe, M., et al. Upregulation of protein-tyrosine nitration in the anterior horn cells of amyotrophic lateral sclerosis. Neurological Research 19, 124-128 (1997). 7.Chou, S.M.,Wang, H.S., and Komai, K. Colocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amyotrophic lateral sclerosis: An immunohistochemical study. Journal of Chemical Neuroanatomy 10, 249-258 (1996). 8.Ferrante, R.J.,Shinobu, L.A.,Schulz, J.B., et al. Increased 3-nitrotyrosine and oxidative damage in mice with a human copper/zinc superoxide dismutase mutation. Annals of Neurology 42, 326-334 (1997). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 稳定性 | ≥ 2 years |
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