| 货号 | 12147S |
| 供应商 | CST |
| 背景 | PD184352 (CI-1040) is a highly potent and selective noncompetitive MEK inhibitor with an IC50 of 17 nM for MEK1 (1). Treatment of cells with PD184352 leads to suppression of MAPK activation (1-4) and potently inhibits the proliferation of cells harboring the B-Raf mutation V600E (2,3). Researchers have shown that PD184352 effectively inhibits MEK5, although not as potently as MEK1 (4). |
| 存放说明 | -20C |
| 参考文献 | 1 . Sebolt-Leopold, J.S. et al. (1999) Nat Med 5, 810-6. 2 . Liu, D. et al. (2007) J Clin Endocrinol Metab 92, 4686-95. 3 . Solit, D.B. et al. (2006) Nature 439, 358-62. 4 . Mody, N. et al. (2001) FEBS Lett 502, 21-4. |
Chemical structure of PD184352.PD184352的化学结构。 | |
Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated or treated with TPA #4174 (200 nM, 20 min) either with or without PD184352 pretreatment (1 hr) at the indicated concentrations, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western blot分析HeLa细胞提取物，血清饥饿过夜，未处理或者先用PD184352 (1小时)根据设计好的不同浓度进行预处理或不预处理，再用TPA #4174 (200 nM, 20分钟)处理，使用的抗体是：Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370（上图）、p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695（下图）。 |
