货号 | 9996L |
描述 | Oligomycin is supplied in lyophilized form.此寡霉素产品为冷冻干粉态。 |
供应商 | CST |
背景 | During oxidative phosphorylation in mitochondria, the electron transport system pumps H+ from the matrix into the intermembrane compartment. This process generates a H+ gradient across the mitochondrial inner membrane. This H+ gradient is used to drive mitochondrial FoF1 ATPase to convert ADP to ATP. Oligomycin, an antibiotic from Streptomyces diastatochromogenes, blocks oxidative phosphorylation by binding to the Fo part of the ATPase to inhibit its activity (1-3). |
存放说明 | -20C |
参考文献 | Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2461-6. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2467-74. Fessenden, J.M. and Racker, E. (1966) J Biol Chem 241, 2483-9. |
Western blot analysis of extracts from 293 cells, untreated (-) or treated with Oligomycin (5 µM, 30 min; +), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 (upper) or AMPKα Antibody #2532 (lower). |