| 货号 | 17460-100uL |
| 描述 | 2,3-Dideoxyadenosine 5-triphosphate (ddATP) is an in vitro inhibitor of reverse transcriptases from retroviruses, including HIV-1 and visna (Kis = 20 and 37 nM, respectively).1,2,3 It also blocks, in vitro, mammalian and bacterial DNA polymerases.4,5 ddATP, produced intracellularly by the phosphorylation of exogenously supplied 2’,3’-dideoxyadenosine, competes with dATP, resulting in chain termination.4,5 Because of this activity, dideoxynucleoside 5’-triphosphates, including ddATP, are used to terminate chain extension produced by the Taq polymerase used in polymerase chain reactions.6 It is also an antagonist of the purinergic receptor P2X2/3(pIC50 = 6.5).7 |
| 别名 | ddATP; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Boyle, N.A.,Rajwanshi, V.K.,Prhavc, M., et al. Synthesis of 2,3-dideoxynucleoside 5-α-P-borano-β,γ-(difluoromethylene)triphosphates and their inhibition of HIV-1 reverse transcriptase. Journal of Medicinal Chemistry 48(7), 2695-2700 (2005). 2.Frank, K.B.,McKernan, P.A.,Smith, R.A., et al. Visna virus as an in vitro model for human immunodeficiency virus and inhibition by ribavirin, phosphonoformate, and 2,3-dideoxynucleosides. Antimicrobial Agents and Chemotherapy 31(9), 1369-1374 (1987). 3.Ueno, T.,Shirasaka, T., and Mitsuya, H. Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase resistant to multiple 2,3-dideoxynucleoside 5-triphosphates. The Journal of Biological Chemisty 270(40), 23605-23611 (1995). 4.Toji, L., and Cohen, S.S. The enzymatic termination of polydeoxynucleotides by 2,3-dideoxyadenosine triphosphate. Proceedings of the National Academy of Sciences of the United States of America 63(3), 871-877 (1969). 5.Yagura, T.,Kozu, T., and Seno, T. Mouse DNA polymerase accompanied by a novel RNA polymerase activity: Purification and partial characterization. Journal of Biochemistry 91(2), 607-618 (1982). 6.Li, Y.,Mitaxov, V., and Waksman, G. Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation. Proceedings of the National Academy of Sciences of the United States of America 96(17), 9491-9496 (1999). 7.Jarvis, M.F.,Bianchi, B.,Uchic, J.T., et al. [3H]A-317491, a novel high-affinity non-nucleotide antagonist that specifically labels human P2X2/3 and P2X3 receptors. Journal of Pharmacology and Experimental Therapeutics 310(1), 407-416 (2004). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥99% |
| 计算分子量 | 475.2 |
| 分子式 | C10H16N5O11P3 |
| CAS号 | 24027-80-3 |
| 稳定性 | ≥ 1 year |
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