| 货号 | 13637S |
| 使用方法 | Camptothecin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 10 mg in 2.87 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 1-24 hr. |
| 供应商 | CST |
| 背景 | Camptothecin is a cytotoxic plant alkaloid originally isolated from C. acuminate that inhibits DNA and RNA synthesis in mammalian cells and is an effective anti-tumor agent (1). Research studies indicate that camptothecin inhibits topoisomerase I with an IC50 of 679 nM (2). Camptothecin binds and stabilizes topoisomerase I–DNA cleavage complexes, which leads to DNA strand breaks (1,3,4). The resultant DNA damage can induce cell cycle arrest in many cancer cell lines (5,6). Inactivation of the tumor suppressor protein p53 can increase the cytotoxicity of camptothecin (6). |
| 存放说明 | -20C |
| 纯度 | >98% |
| 计算分子量 | 348.4 g/mol |
| 参考文献 | 1 . Hsiang, Y.H. et al. (1985) J Biol Chem 260, 14873-8. 2 . Luzzio, M.J. et al. (1995) J Med Chem 38, 395-401. 3 . Marchand, C. et al. (2006) Mol Cancer Ther 5, 287-95. 4 . Jaxel, C. et al. (1991) J Biol Chem 266, 20418-23. 5 . Jaks, V. et al. (2001) Oncogene 20, 1212-9. 6 . Gupta, M. et al. (1997) Clin Cancer Res 3, 1653-60. |
Chemical structure of camptothecin. | |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Camptothecin (6 hr) at the indicated concentrations, using Phospho-p53 (Ser15) Antibody #9284 (upper), p53 (1C12) Mouse mAb #2524 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
