FKBP-12 Pure 2C1-87 100ug
货号：
554091 基本售价：
5282.0 元 规格：
100ug
产品信息
概述| 货号 | 554091 |
| 描述 | FKBP-12 (for FK506 binding protein-12 kDa) is a ubiquitously expressed protein involved in T-cell signaling that binds to FK506. FK506 belongs to a class of proteins that have peptidyl-prolyl cis-trans isomerase (PPIase) activity. This class of proteins is thought to be involved in folding proteins and peptides into their native conformations. It is thought that peptidyl-prolyl isomerization may play a role in regulating components of the T-cell signal transduction pathway such as transcription factors, protein kinase activation, and ion channel function. FK506, like cyclosporin, is an agent that inhibits T- and B-cell early activation processes that are key to an effective immune response. In T cells these agents disrupt a step in the signal transduction pathway from the T-cell receptor to cytokine genes involved in coordinating the immune response. In particular, they seem to disrupt pathways that induce a measurable rise in Ca2+. NFAT, a transcription factor essential for early T-cell activation, appears to be a specific target of FK506 and cyclosporin because transcription directed by NF-AT is specifically blocked in T cells treated with these agents. NFAT is formed when a signal from the T-cell antigen receptor induces a pre-existing cytoplasmic subunit to translocate to the nucleus and combine with a newly synthesized nuclear subunit of NFAT. When FK506 binds to FKBP-12, the complex blocks translocation of the cytoplasmic subunit of NF-AT into the nucleus by inhibiting a Ca2+-dependent phosphatase. Since the cytoplasmic subunit is not translocated into the nuclear, the formation of NFAT and its subsequent transcriptional activity is blocked. The reduced molecular weight of FKBP-12 is 12 kDa. The 2C1-87 antibody recognizes FKBP-12. Specifically it has been shown to recognize human, mouse, and rat FKBP-12. Recombinant FKBP-12 was used as immunogen. The antibody was originally characterized by biochemical, immunohistochemical, and functional assays. |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) Mouse, Rat (Reported) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IHC(P) |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Recombinant FKBP-12 |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
