| 货号 | HCA266 |
| 克隆号 | AbD23895_hIgG1 |
| 同种亚型 | IgG1 |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Human anti panitumumab specificity ELISA A microtiter plate was coated over night with various antigens at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23895_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit® | |
Human anti panitumumab specificity titration ELISA A microtiter plate was coated over night with panitumumab, human IgG2/kappa, adalimumab or human EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti panitumumab (clone AbD23895_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®.Data for binding to panitumumab is shown as the mean of three measurements | |
Panitumumab bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated over night with Human anti panitumumab (clone AbD23894, HCA264) at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of panitumumab. Detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23895_hIgG1, HCA266) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit® | |
Human anti panitumumab bridging ELISA for Anti-Drug Antibody (ADA) assay development A microtitre plate was coated over night with panitumumab at a concentration of 1 μg/ml (black line) or 0.5 μg/ml (red line). After washing and blocking with PBST+5% BSA, 10% Human serum was added spiked with increasing concentrations of Human anti panitumumab HCA265 (clone AbD23897_hIgG1) or HCA266 (clone AbD23895_hIgG1). Detection was performed using HRP-conjugated panitumumab. Data is shown as the mean of three measurements | |
Human anti panitumumab inhibits the binding of panitumumab to EGFR A microtiter plate was coated over night with EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of panitumumab (0.3 μg/ml) plus increasing concentrations of Human anti panitumumab (clone AbD23895) in the monovalent Fab format was added. Free panitumumab, still capable of binding to the human EGFR coated plate, was detected using Goat anti Human IgG F(ab)2 HRP (0500-0099) labeled antibody and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements |
