| 货号 | MCA2312F |
| 克隆号 | BL1H7 |
| 同种亚型 | IgG1 |
| 反应种属 | Pig |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of porcine peripheral blood mononuclear cells with FITC conjugated Mouse anti Porcine CD172a antibody, clone BL1H7 (MCA2312F) | |
Published customer image: Mouse anti Porcine CD172a antibody, clone BL1H7 used to evaluate SWC3 expression on bone marrow derived dendritic cells by flow cytometry. Image caption: Single colour flow cytometry analysis of changes in several immunologically relevant cell surface molecules of bone marrow-derived dendritic cells after 48 h of incubation with different PRRSV strains at a multiplicity of infection of 0.01 (as determined by titration in porcine alveolar macrophages). Grey histograms show cells stained with an irrelevant isotype-matched antibody and a secondary FITC or phycoerithrin conjugated antibody. Blue histograms show uninfected cells and red line histograms correspond to PRRSV-infected cells. Averages and variation coefficients are included. Grey values show the uninfected cell parameters and black values correspond to PRRSV-infected cells. A) SLA-I, SLA-II, CD80/86, CD14; B) SwC3, SwC9, CD163, CD16. From: Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E. Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates. Vet Res. 2011 Jan 18;42:9. | |
Published customer image: Mouse anti Porcine CD172a antibody, clone BL1H7 used to assess CD172a expression on porcine monocytes by flow cytometry. Image caption: Chemokine receptor mRNA expression in monocyte subsets. (A) Dot plot of Tük4 versus SLA-II expression in monocytes obtained from PBMC (20 000 cells were acquired). Three subsets can be identified: Tük4+SLA-II-, Tük4+SLA-II+, and Tük4-SLA-II+. (B) The Tük4+SLA-II- and Tük4-SLA-II+ monocyte subsets were magnetically isolated as described in Materials and methods section, and the expression of the indicated markers was analysed by flow cytometry (5 000 cells acquired). (C) Total RNA isolated from PBMC, the whole monocyte population (CD172a+) or the Tük4+SLA-II- and Tük4-SLA-II+ monocyte subsets shown in (B) were reverse transcribed, and the cDNA amplified by PCR with specific primers for GAPDH or different chemokine receptors. PCR products were analysed by 1.5% agarose gel electrophoresis. Data are representative from three independent experiments using different donors. From: Moreno S, Alvarez B, Poderoso T, Revilla C, Ezquerra A, Alonso F, Dominguez J. Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2. Vet Res. 2010 Sep-Oct;41(5):76. | |
Published customer image: Mouse anti Porcine CD172a antibody, clone BL1H7 used to identify CD172a expressing porcine monocytes for subsequent analysis of monocyte subsets based on CD14 and SLA II expression by flow cytometry. Image caption: Differential binding of CCL2-GFP to monocyte subsets. Porcine PBMC were incubated with CCL2-GFP (CHO cell supernatant) followed by Alexa 488-conjugated rabbit anti-GFP IgG, and then double stained for Tük4 and SLA II. The histograms are gated on the appropriate monocyte subpopulation regions as defined in the dot-plots. Grey histograms show the background fluorescence of monocytes incubated with supernatants from CHO cells transfected with the InvCCL2-GFP construct. 25 000 cells were acquired. From: Moreno S, Alvarez B, Poderoso T, Revilla C, Ezquerra A, Alonso F, Dominguez J. Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2. Vet Res. 2010 Sep-Oct;41(5):76. |
