| 货号 | MCA2228F |
| 克隆号 | 49.1 |
| 同种亚型 | IgG2a |
| 反应种属 | Sheep |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine MHC CLASS II DQ/DR (POLYMORPHIC) (MCA2228F) | |
Published customer image: Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence. Image caption: Primary infection of nasopharyngeal mucosal epithelial cells is similar in non-vaccinated and vaccinated cattle. Microscopic distribution of FMDV structural (VP1) and non-structural (3D) protein in dorsal nasopharyngeal mucosa of non-vaccinated (A-C) and vaccinated (D-F) cattle at 24 hpi. Multichannel immunofluorescent technique. A) Nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). FMDV VP1 (red) and 3D (aqua) proteins co-localize with cytokeratin (green) in foci of primary FMDV infection in superficial layer of follicle-associated epithelium. Scarce MHC II+ cells (purple) are present in the subepithelium. 20x magnification, scale bar 50μm. B-C) 40x magnification of region identified in (A). B) Select channel combinations demonstrating co-localization of FMDV VP1(red) and FMDV 3D (aqua) with cytokeratin (green), but not with MHC II (purple) C) Merge of all images shown in (B), scale bar 25μm. D) Nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). FMDV VP1 (red) and 3D (aqua) proteins co-localize with cytokeratin (green) within follicle associated epithelium. Intra-epithelial MHC II+ cells (purple) are in close proximity to virus-infected epithelial cells. 20x magnification, scale bar 50μm. E-F) 40x magnification of region identified in (D). E) Select channel combinations demonstrating FMDV VP1 (red) and 3D (aqua) colocalization with cytokeratin (green)-expressing cells and with MHC II- expressing cells (purple). E) Merge of images shown in (F), scale bar 25μm. From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666. | |
Published customer image: Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence. Image caption: Differential host-virus interactions in nasopharyngeal mucosa of non-vaccinated (A-C) versus vaccinated (D-F) cattle. Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). Host cells expressing MHC II (purple) and/or CD11c (aqua) are present in the subepithelial compartment and interspersed within epithelium, but without co-localizing with viral protein. 10x magnification, scale bar 100μm. B-C) 40x magnification of region identified in (A), scale bars 25μm. B) Select channel views demonstrate lack of co-localization of FMDV VP1 (red) with CD11c (aqua)/ MHC II (purple)-double positive cells. C) Merge of all images shown in (B), scale bar 25 μm. D) FMDV VP1 (red) protein co-localizes with cytokeratin (green) in an epithelial erosion in dome region of follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). Regionally extensive infiltration by MHC II (purple) and/or CD11c (aqua)-expressing cells co-localizing and interspersing with viral antigen.10x magnification, scale bar 100μm. E-F) 40x magnification of region identified in (D), scale bar 25μm. E) Select channels demonstrating co-localization of FMDV VP1 (red) with CD11c (aqua), MHC II (purple). F) Merge of images shown in (E), scale bar 25 μm. From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666. | |
Published customer image: Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence. Image caption: FMDV co-localizes with cytokeratin and MHCII/CD11c-expressing cells in palatine tonsil of non-vaccinated steer at 72hpi. A) FMDV VP1 (red) predominantly in cytokeratin-positive cells of epithelial crypt of palatine tonsil of non-vaccinated steer (animal number 9) during viremia and clinical FMD at 72 hpi. Abundant cells expressing MHC II (purple) and/or CD11c (aqua) interspersed with epithelial cells. 10x magnification, scale bar 100 μm. B) Select channels of image in (A). FMDV VP1 (red), cytokeratin (green), CD11c (aqua), MHC II (purple) demonstrate FMDV antigen is exclusively within epithelial compartment. C) High magnification of region identified in (A). Co-localization with FMDV VP1within subset of MHC II (purple) and/or CD11c (aqua) expressing cells within crypt epithelium (arrows in C & D). 40x magnification, scale bar 25 μm D) Individual channels of image in (B). FMDV VP1 (red), cytokeratin (green), CD11c (aqua), MHC II (purple). From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666. |
