| 货号 | MCA1658 |
| 克隆号 | 1D4 |
| 同种亚型 | IgG1 |
| 反应种属 | Sheep |
| 来源宿主 | Mouse |
| 应用 | C, E, F*, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Rabbit anti Sheep interleukin 1β antibody (AHP423) used for the detection of bound interleukin 1β from lamb sera in an ELISA using Mouse anti Sheep IL-1&beta antibody, clone 1D4 as a capture antibody (MCA1658). For IL-6, capture is with Mouse anti Sheep IL-6 antibody, clone 4B6 (MCA1659) and detection using Rabbit anti Sheep IL-6 antibody (AHP424). Subsequent visualisation uses HRP conjugated Goat anti Rabbit IgG in both cases. Image caption: Systemic and diaphragm cytokine responses. Plasma IL-1β (A) and IL-6 (B) protein content. Values are mean ± SEM. Diaphragm IL-1β (C) and IL-6 (D) mRNA expression. Values are median (with 10th and 90th centiles). * p<0.05 compared to Sal/Sal; # p<0.05 compared to Sal/LPS. From: Karisnan K, Bakker AJ, Song Y, Noble PB, Pillow JJ, et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs. PLoS ONE 10(4): e0124390. | |
Published customer image: Mouse anti Ovine IL-1β antibody, clone 1D4 (MCA1658) used for the determination of IL-1β signal in equine follicular fluid samples following gonadotrophin administration by western blotting. Image caption: Content of IL-1β in follicular fluid at different stages: early dominance [ED], end of growth [T0 h], 6 h [T6 h], 12 h [T12 h], 24 h [T24 h] and 34 h [T34 h] after injection of crude equine gonadotropin A) Western immunoblots using a mouse anti-ovine IL-1β. Lane 1, early dominance [ED]; lane 2, end of growth [T0 h]; lanes 3, 4, 5 and 6, 6 h [T6 h], 12 h [T12 h], 24 h [T24 h], and 34 h [T34 h] after CEG injection respectively. Lanes P1 and P2, positive controls (ovine follicular fluids). B) Quantification of IL-1β signals visualized in follicular fluid at different stages as described above. IL-1β signal in each lane was measured by ImageQuant software and is expressed per follicle. Results are expressed in arbitrary units and represent means ± SEM of four to seven samples. a, b, and c are significantly different (p < 0.05 at least) according to the non parametric Wilcoxon Mann Whitney test. The data presented are representative of four experiments (electrophoresis and immunoblotting). From: Martoriati A, Gérard N. Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare. Reprod Biol Endocrinol. 2003 May 16;1:42. Copyright © 2003 Martoriati and Gérard; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. | |
Published customer image: Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1β antibody (MCA1658) as a capture reagent Image caption: Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-○-) and S. simulans (-●-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL. From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulansandS. epidermidis. Vet Res. 2011 Mar 17;42:49. | |
Published customer image: Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1&beta antibody (MCA1658) as a capture reagent Image caption: Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-□-) and spontaneously eliminated (-■-) S. epidermidisandS. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL. From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulansandS. epidermidis. Vet Res. 2011 Mar 17;42:49. |
