| 货号 | MCA2216F |
| 克隆号 | 38.65 |
| 同种亚型 | IgG2a |
| 反应种属 | Sheep |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Reconstitute with 1 ml distilled water |
| 运输条件 | |
| 存放说明 | Prior to reconstitution store at +4oC. Following reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine CD8:FITC (MCA2216F) | |
Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine CD8 MCA2216GA) followed by Rabbit F(ab')2 ANTI MOUSE IgG:FITC (STAR9B) | |
Published customer image: Mouse anti Bovine CD8 antibody, clone 38.65 used to demonstrate the presence of CD8 expressing T cells in ovine placenta during gestation by immunohistochemistry on cryostat sections. Image caption: Comparison of the immunohistochemical labelling of T inflammatory cells and parasite antigen in the placenta. This panel compares the distribution and frequency of T lymphocytes (CD3, CD4 and CD8 antigens) and parasite antigen immunohistochemically labelled in samples from the three groups. Pictures showing the labelling of T cells (first three rows) were taken at 75× and those of the parasite antigen (last row) were taken at 150×. F: foetal mesenchyme, M: maternal endometrial stalk. From: Arranz-Solís D, Benavides J, Regidor-Cerrillo J, Horcajo P, Castaño P, Del Carmen Ferreras M, Jiménez-Pelayo L, Collantes-Fernández E, Ferre I, Hemphill A, Pérez V, Ortega-Mora LM. Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation. Vet Res. 2016 Jan 6;47(1):2. | |
Published customer image: Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine Peyer's patch and jejenum by flow cytometry and immunofluorescence. Image caption: CD8+ lymphocyte increase in the jejunum and jejunal Peyer's patches. (A) Cells isolated from jejunal Peyer's patches (JPP) and jejunum were double labelled with anti CD8 and NCR1 mAbs and analyzed by flow cytometry. The plots show data from a 6 dpi inoculated lamb and its 7 day-old matched control and are representative of 8 matched pairs of 7 day-old lambs. The gating used for the analyses shown in B is indicated with the corresponding colours. (B) The percentages of the 3 different populations of CD8+ lymphocytes corresponding to the 3 gates shown in (A) were analyzed in jejunum and JPP from lambs 2–6 dpi. Control lambs (open symbols), inoculated lambs (filled symbols). The means are indicated in red. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with * p?<?0.05, ** p?<?0.01, *** p?<?0.001. (C-D) Section of an ileal Peyer's patch (IPP) from a 7 day old control (C) and a 6 dpi lamb (D) were double labelled with anti CD8 (green) and Ki-67 (blue) antibodies. The images shown are obtained by merging these images with the white light image. The double positive cells (arrow) display CD8 cytoplasmic staining and Ki67 nuclear staining. Pinpoint sized spots (arrowhead) are identified as autofluorescence. Dome (d), lamina propria (lp). Bar 50 μm. From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment". Veterinary Research 2015 46:28. | |
Published customer image: Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine Peyer's patch and jejenum by immunofluorescence. Image caption: Significant increase of CD8+ cells in the ileum of infected lambs. Ileal Peyer's patches sections from a pair of 6 day-old matched lambs, control (A) and inoculated (B), were labelled with Ab against CD3 (red), CD8 (green) and γδTCR (blue). Increased numbers of yellow CD3+/CD8+ cells (arrow) in the lamina propria (lp) and dome (d) were seen in the inoculated animals compared with the controls. Few or no CD3+/CD8+/γδTCR+ cells were observed. Light blue dots (arrowhead) were identified as autofluorescence and thus differentiated from the specific labelling which was localised in the cell membrane. Absorptive epithelium (ae), follicle-associated epithelium (fae), follicle (f), interfollicular area (ifa). Bar 50 μm. (C) The increase of CD3+/CD8+ cells in the ileal Peyer's patch was demonstrated by quantitative analysis of positive cells per mm2 in villi and dome, including the covering epithelium. The graphs show the median with 95% confidence interval constructed using the Bernoulli-Wilcoxon procedure. Statistically significant differences are indicated as ** p?< 0.01 and *** p?<?0.001. From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment". Veterinary Research 2015 46:28. | |
Published customer image: Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine jejenum by flow cytometry. Image caption: Perforin+?lymphocytes among CD8+ lymphocytes in the gut.(A) Cells extracted from gut tissues were double labelled with anti NCR1 and CD8 mAbs then fixed and permeabilized and labelled with an anti perforin mAb. The plot shows (example of jejunum at 6 dpi) the gating of the lymphocyte populations analyzed. (B) Lymphocytes from an inoculated lamb (3 dpi) and its control were gated either on the CD8+/NCR1+ or the CD8tot/NCR1- populations then analyzed for the perforin content. The red line limited histogram corresponds to the inoculated lamb (In), the black line limited histogram to its matched control (Co) and the gray filled histogram to the isotype control of the perforin mAb (iso) and the mean fluorescence intensity (MFI) is indicated for each histogram. (C) The analysis of the perforin expression by CD8+ lymphocyte sub-populations (gates shown on Figure 7A) was performed in 3 control and 4 age-matched 6 dpi inoculated lambs. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with ** p?<?0.01 and *** p?< 0.001. From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment". Veterinary Research 2015 46:28. | |
Published customer image: Mouse anti Sheep CD8 antibody, clone 38.65 used for the detection of CD8 expressing lymphocytes in ovine jejenum by flow cytometry. Image caption: Expression of CD25 on small intestinal NCR1+ and CD8+ lymphocytes. (A) The expression of the activation marker CD25 was analyzed at 6 dpi in the populations gated as indicated. The individual percentages (B) and the mean fluorescence intensity (MFI) of CD25+ cells (C) are shown for the 3 populations. Medians are shown with red bars. Control lambs (open symbols), inoculated lambs (filled symbols). In C, the results of the two experiments are shown with red or black symbols. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with ** p?<?0.01 and *** p?<?0.001. From: Olsen et al. "The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1- CD8+ cell recruitment". Veterinary Research 2015 46:28. |
