| 货号 | MCA72G |
| 克隆号 | YD1/63.4.10 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Rat |
| 应用 | C*, F |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Rat anti Human HLA-DR antibody, clone YD1/63.4.10 used for the evaluation of MHC class II expression on human PBMC by flow cytometry. Image caption: Plasmacytoid dendritic cells show weak T cell stimulatory capacity. A: Flow cytometric analysis of human PBMC and DC. pDC are characterized as lineage- CD11c- CD303+ cells (middle panel). Dot plots (right side) exemplarily depicts isolated pDC, percentage indicates purity. Histograms show surface expression of CD40, CD80, CD86, CD58 and HLA-DR on resting and CpG/IL-3-activated pDC and cytokine cocktail activated cDC. Isotype-matched control mAb staining is shown in grey. One representative result of three independent experiments is shown. B: T cell stimulatory capacity of activated pDC and cDC. CD4+ or CD8+ Teff were stimulated with allogeneic pDC or cDC at different DC:Teff ratios. Teff proliferation was determined on day 4 of culture by incorporation of 3H-Tdr and presented as mean ± SD of triplicates. One representative experiment out of 3 is shown. C: Expansion of DC-stimulated Teff. Expansion rates of CD4+ or CD8+ Teff after stimulation with allogeneic cDC (n = 4) or pDC (n = 2), determined at day seven. The figure shows the relative fold expansion of initially stimulated Teff. Bar indicates mean values. D: Cytokine profiles of Teff seven days after stimulation with pDC or cDC. Numbers represent the percentage of cytokine-positive Teff. One typical result out of six is shown. ** p<0.01. From: Hubo M, Jonuleit H (2012) Plasmacytoid Dendritic Cells Are Inefficient in Activation of Human Regulatory T Cells. PLoS ONE 7(8): e44056. |
