| 货号 | FAB7080G |
| 别名 | CD8; CD8A |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects cotton rat CD8 alpha in direct ELISAs. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 925 (Human); 12525 (Mouse); 24930 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NIH‑3T3 mouse embryonic fibroblast cell line transfected with cotton rat CD8 alpha and SP2/O mouse melanoma cell line transfected with cotton rat CD8 alpha. Accession # AAL55392 |
| 生物活性 | Cotton Rat |
| 标记 | Alexa Fluor 488 |
| 背景 | CD8 alpha (CD8 alpha ; also OX8, Leu-2 and Lyt-2) is a 23 kDa (predicted) member of the Ig superfamily of molecules. It is found on immature thymocytes, cytotoxic and suppressor T cells, select mast cells, and splenic and bone marrow dendritic cells (in rodent). On T cells, CD8 alpha exists as either a disulfide linked homodimer, or heterodimer bound to CD8 beta. CD8 alpha beta is best known as a coreceptor for the TCR, enhancing TCR signaling. CD8 alpha alpha serves a different function and acts as a TCR corepressor that blocks T cell activation. Based on rat, mature Sigmodon hispidus/cotton rat CD8 alpha is a 210 amino acid (aa) type I transmembrane protein that consists of a 161 aa extracellular region (aa 24-184) with one V-type Ig-like domain (aa 35-135), and a 30 aa cytoplasmic tail (aa 206-235). Although Sigmodon hispidus is called a rat, it is not. It is a rodent, and rat CD8 alpha is the closest ortholog to cotton rat CD8 alpha currently reported. Over the extracellular region, rat and cotton rat CD8 alpha share only 54% aa identity. Rat CD8 alpha runs at 34-39 kDa in SDS-Page, and possesses one utilized N-linked and O-linked glycosylation site (vs. no N-linked sites in cotton rat CD8 alpha ). Based on this organization, and assuming O-linked glycosylation, cotton rat CD8 alpha would be expected to run at about 30-34 kDa in SDS-PAGE. Over aa 24-182, cotton rat CD8 alpha shares 54% and 48% aa identity with rat and mouse CD8 alpha, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of CD8 alpha in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD8 alpha Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7080G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
