| 货号 | MCA647G |
| 克隆号 | MK 1 A6 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | C*, E, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Detection of Human Fc (CH2) using Mouse anti Human Fc specific:HRP antibody (MCA647P) in an Adalimumab sensitivity ELISA. Human anti Adalimumab (HCA202) was used as the coating antibody followed by Adalimumab (D2E7) at the given concentrations as the antigen | |
Published customer image (A) Selection strategy for the generation of pH-dependent binders. A yeast displayed Fcab library was incubated with 3 nM biotinylated Her2-ECD, followed by labeling with SA-PE and anti-CH2-FITC. The incubations were either performed at pH 7.4 (selection rounds 1, 2, and 5) or at pH 6.0 (rounds 3, 4, and 6). If the staining procedure was done at pH 7.4, the cells were subsequently sorted for binding to Her2, whereas incubation at pH 6.0 was followed by selection of non-binders. Exemplarily, the dot plots from selection rounds 4 (top) and 5 (bottom) are shown. The boxes within the plots indicate the gates that were used for sorting. (B) Analysis of the pH-dependence of Her2-binding of selected Fcab mutants. After six rounds of flow cytometric sorting, individual Fcab-clones were analyzed. Fcabs were displayed on yeast and incubated with 3 nM biotinylated Her2, followed by detection of antigen binding with SA-PE and of the surface display level with anti-Xpress-APC (recognizing an N-terminal expression tag located between Aga2p and the Fcab). Incubation steps were either performed at pH 6.0 (gray lines) or 7.4 (black lines). Only Xpress-positive (i.e. displaying) cells were analyzed. In total, 10 Fcabs that have been enriched during selection were tested for pH-dependent Her2 binding. Only the three best performing clones (P1, P2, and P3) are shown. From: Traxlmayr, M. W., Lobner, E., Hasenhindl, C., Stadlmayr, G., Oostenbrink, C., Rüker, F. and Obinger, C. (2014), Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution. Biotechnology Journal, 9: 1013–1022. |
