| 货号 | MCA2044F |
| 克隆号 | MEM-G/9 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Mouse anti Human HLA-G antibody, clone MEM-G/9 used for the evaluation of HLA-G expression on transfected cells by flow cytometry. Image caption: MiR-148a and miR-152 down-regulate HLA-G expression and reduce LILRB1 binding. 721.221/HLA-G (G variant, A, or C variant, B) were transduced with the control miRNA, miR-148a or miR-152. The various cells were then stained with anti HLA-G mAb and analyzed by FACS. One out of three representative experiments is shown. (C) FACS histograms re HLA-G staining of 721.221/HLA-G cells not expressing the 3′UTR of HLA-G. One out of three representative experiments is shown. (D–E) LILRB1-Ig staining of 721.221/HLA-G (G variant, D, or C variant, E) and of 21.221/HLA-G cells not expressing the 3′UTR of HLA-G (F) transduced with control miRNA, with miR-148a or with miR-152. Black histogram: cells transduced with a control miRNA. Dark grey histogram: cells transduced with miR-148a. Light grey histogram: cells transduced with miR-152. One out of three representative experiments is shown. (G) Quantitative real-time PCR analysis of HLA-G mRNA levels in 721.221/HLA-G cells (G variant), presented relative to hUBC. One out of three representative experiments is shown. From: Manaster I, Goldman-Wohl D, Greenfield C, Nachmani D, Tsukerman P, Hamani Y, et al. (2012) MiRNA-Mediated Control of HLA-G Expression and Function. PLoS ONE 7(3): e33395. | |
Published customer image: Mouse anti HLA-G antibody, clone MEM-G/9 used for the evaluation of cuirculating HLA-G in Primary Antiphospholipid Syndrome and control patients by ELISA. Image caption: HLA-G serum levels in 44 PAPS patients and 43 healthy controls, and in PAPS patients with and without heparin. From: de Carvalho JF, de Oliveira RM, Rodrigues CE, Glezer A, Bonfá E, Pereira RM. Heparin increases HLA-G levels in primary antiphospholipid syndrome. Clin Dev Immunol. 2012;2012:232390. | |
Published customer image: FITC conjugated Mouse anti Human HLA-G antibody, clone MEM-G/9 (MCA2044F) used for the demonstration of HLA-G expression on placental trophoblasts by immunofluorescence Image caption: Dual immunofluorescence using HLA-G (an extravillous trophoblast marker) and anti-M30 cytodeath reveals that trophoblast apoptosis is markedly increased in spontaneous abortions. From: Guenther S, Vrekoussis T, Heublein S, Bayer B, Anz D, Knabl J, Navrozoglou I, Dian D, Friese K, Makrigiannakis A, Jeschke U. Decidual macrophages are significantly increased in spontaneous miscarriages and over-express FasL: a potential role for macrophages in trophoblast apoptosis. Int J Mol Sci. 2012;13(7):9069-80. | |
Published customer image: FITC conjugated Mouse anti Human HLA-G antibody, clone MEM-G/9 used for the identification of HLA-G expressing trophoblast cells by flow cytometry. Image caption: The IL-2 receptor β subunit is up-regulated on trophoblast cells as they differentiate from villous to extravillous phenotype. Upregulation of IL-2Rβ expression was validated by flow cytometry (A–C) and immunohistochemistry (D). Preparations of placental cells from normal first trimester pregnancies were gated on scatter, leukocytes excluded by CD45 labelling (gate R2) and subunits of the IL-15 receptor complex stained on villous (EGF-R+) or extravillous (HLA-G+) trophoblast cells (A). Dot plots are shown of IL-2Rβ subunit staining villous (B) and extravillous trophoblast (C). Shown here are cells from the same placenta which is representative of 3 pregnancies analysed. The IL-2Rβ was also localised histologically in sections of implantation site from the first trimester (D). The low power plan stained with cytokeratin shows villous mesenchyme (top left) and a column of EVT (bottom right) developing from placental villi (centre). Higher power pictures show strong IL-2Rβ staining detected on extravillous trophoblast migrating away from the villi, whereas staining was negligible on villous trophoblast and villous mesenchymal cells. From: Apps R, Sharkey A, Gardner L, Male V, Trotter M, Miller N, North R, Founds S, Moffett A. Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells. Placenta. 2011 Jan;32(1):33-43. |
