货号 | 7263-1006 |
克隆号 | 3F6B3 (10H6) |
同种亚型 | IgG1 |
反应种属 | Bacterial |
来源宿主 | Mouse |
应用 | C, E, P* |
供应商 | Bio-Rad Antibodies |
运输条件 | |
存放说明 | Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。 |
Published customer image: Mouse anti peptidoglycan antibody, clone 3F6B3 (7263-1006) used for the detection of Bacillus cereusorStaphylococcus epidermidis derived biofilms by innunofluorescence. Image caption: verlay and fluorescent images of B. cereus (A, B), S. epidermidis (C, D) biofilms, B. canadensis (E, F) and T. rex (G, H) vessels, exposed to antibodies raised against peptidoglycan, a bacterially produced glycosaminoglycan that is a component of both bacterial cell walls and the EPS they secrete. Binding of these antibodies is evident in both biofilm products, but no binding is seen of these antibodies to either dinosaur vessel. Scale bar for all images = 20 μm From:Citation: Schweitzer MH, Moyer AE, Zheng W (2016) Testing the Hypothesis of Biofilm as a Source for Soft Tissue and Cell-Like Structures Preserved in Dinosaur Bone. PLoS ONE 11(2): e0150238. doi:10.1371/journal.pone.0150238 | |
Published customer image: Mouse anti Peptidoglycan antibody used to detect spirochetes in the brain of a patient with Lyme neuroborreliosis by immunofluorescence. Extra- and intracellular atypical and cystic forms of spirochetes in the cerebral cortex of a patient with pathologically and serologically confirmed chronic Lyme neuroborreliosis where Borrelia burgdorferi sensu stricto was cultivated from the brain. A: Colony-like agglomeration of spirochetes as revealed by monoclonal anti-OspA antibody in the cerebral cortex. B: A close up of the central part of the mass seen in A. In addition to a few helically shaped spirochetes (arrow) numerous ring-shaped forms and spherules (asterisk) are visible, which are identical to those observed in vitro following 1 week Borrelia exposure of primary neurons (compare with Fig. 4G). C: Spirochetes showing loop or ring-shaped formations (arrows) in the cerebral cortex immunostained with a polyclonal anti-Borrelia burgdorferi antibody (Biodesign, B65302R). They are similar to those of Treponema pallidum (arrows in D) observed in the cerebral cortex of a patient with general paresis. Immunostaining was performed using a polyclonal anti-Treponema pallidum antibody (Biodesign, B65210R). E: Helically shaped OspA immunoreactive spirochetes in the cytoplasm of a cortical pyramidal neuron. In addition to one more typical form (arrow), fine OspA positive minute granules along filamentous forms are seen. F: Intracellular ring-shaped forms (arrow) showing positive immunoreaction with a polyclonal anti-Borrelia burgdorferi antibody (BB1017). They are identical to those observed in chicken primary neurons infected with Borrelia (compare F with Figure 4D). Near the asterisk a large strongly immunoreactive cyst form is visible. Spirochete forming loop in the cerebral cortex (G) and in the cytoplasm of an epithelial cell of the choroid plexus (H) are seen as visualized by anti-OspA and anti-bacterial peptidoglycan antibodies, respectively. I: A similar atypical spirochete forming loops in the cerebral cortex as visualized with Thioflavin S. J: In an area with colony-like spirochete aggregation in addition to some typical, regularly coiled Borrelia spirochetes (arrow) OspA positive cystic forms (asterisk) are seen. K: In the cerebral cortex near the colony-like spirochetal agglomerate a spirochete cyst (asterisk) similar to that observed in vitro is visible (compare it with Figure 5 G-J). Immunostaining was performed using a monoclonal anti-OspA antibody. Bars: A = 20 μm; B-J = 10 μm, K = 5 μm. From: Miklossy J, Kasas S, Zurn AD, McCall S, Yu S, McGeer PL. Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme neuroborreliosis. J Neuroinflammation. 2008 Sep 25;5:40. |