| 货号 | MCA5792 |
| 克隆号 | Staph11-232.3 |
| 同种亚型 | IgG3 |
| 反应种属 | Bacterial |
| 来源宿主 | Mouse |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Mouse anti Staphylococcus aureus antibody, clone Staph11-232.3 (MCA5792) used as a capture reagent in an ELISA to evaluate linear peptide binding. Image caption: Both SP27 and MAP27' bind to anti-PGN mAb specifically. (A) The sequences of SP27 (Biotin-SASPHHHSRLRSESGG) and SP27' (Biotin-SPHHHSRLRSESSAGG). Underlined letters represent nonspecific flanking amino acids. (B) Both SP27 and SP27' bind to anti-PGN mAb in a dose-dependent manner. (C) Both SP27 and SP27' (100 µg/ml) specifically bind to anti-PGN mAb, but not to anti-LTA mAb. For ELISA assays in panel B and C, anti-PGN mAb or anti-LTA mAb was used to coat the wells at a concentration of 5 µg/ml, SP27, SP27' or non-specific peptide L2 (in panel B, sequence: Biotin-HSGHWDFRQWWQPSGG) was then added at indicated concentrations and incubated at 37°C for 40 min, followed by detection with HRP-labeled streptavidin. (D) The structure diagram of MAP27. MAP27 was synthesized in a tetra-branched form that contains four copies of a sequence (SASPHHHSRLRSESGG) that mimics PGN epitope. (E) MAP27 binds to anti-PGN mAb specifically. (F) MAP27 binds to anti-S. aureus polyclonal antibodies specifically. For ELISA assays in panel E and F, MAP27 or MAPctrl was used to coat the wells of a microplate. Anti-PGN mAb or anti-S. aureus polyclonal antibodies was added, followed by detection with HRP-labeled antibodies. The absorbance was measured at OD450nm. The results are shown as means ±SEM. * P<0.05, ** P<0.01, *** P<0.001. From: Wang X-Y, Huang Z-X, Chen Y-G, Lu X, Zhu P, Wen K, et al. (2015) A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with Staphylococcus aureus. PLoS ONE 10(8): e0136888. |
