| 货号 | MCA2466 |
| 克隆号 | 14B7 |
| 同种亚型 | IgG2a |
| 反应种属 | Viral |
| 来源宿主 | Rat |
| 应用 | IF, IP, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Rat anti Epstein-Barr Virus LMP2A antibody, clone 14B7 (MCA2466) used for the detection of LMP2A in cell extracts by western blotting. Epstein-Barr virus nuclear antigen (EBNA1) is detected using Mouse anti EBNA1 antibody, clone E1-2.5 (MCA2707). Image caption: (a) Epitope comparison amongst the variants and those observed in the 3 cell lines studied. Red letters denote amino acid mutation. (b) Quantitation of HLA-A0201/LMP1,/LMP2A and/EBNA1 on CCRF-SB, RPMI-6666 and C666-1A2 using TCR-like mAbs based on flow cytometry. Error bars are standard deviation from an average of three independent experiments. **p<0.01. n.d. denotes not detectable. (c) LMP1, LMP2A and EBNA1 in each of the cell lines was determined using RT-PCR and Western blot. β-actin was used as control. From: Sim AC, Too CT, Oo MZ, Lai J, Eio MY, Song Z, Srinivasan N, Tan DA, Pang SW, Gan SU, Lee KO, Loh TK, Chen J, Chan SH, MacAry PA. Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies. Sci Rep. 2013 Nov 18;3:3232. | |
Published customer image: Rat anti Epstein-Barr Virus LMP2A antibody, clone 14B7 (MCA2466) used for the detection of LMP2A in cell extracts by Immunofluorescence and immunohistochemistry. Epstein-Barr virus nuclear antigen (EBNA1) is detected using Mouse anti EBNA1 antibody, clone E1-2.5 (MCA2707). Image caption: Generation of Hu-NSG mice EBV model and staining of infected mouse spleen and clinical NPC biopsies with TCR-like mAbs. (a) Experimental workflow of the Hu-NSG mice EBV model -isolation of HSC; injection into NSG mice; flow cytometric analysis of reconstitution; EBV infection of Hu-NSG mice; and development of splenic lesions. The reconsitution of the Hu-NSG mice with human immune cells was approximately 65% ± 3.9%. (b) Spleens of EBV infected HLA-A0201 Hu-NSG mice showed positive red staining for TCR-like mAbs specific to LMP1, LMP2A and EBNA1 binding. Scale bars, 50 μm. (c) Immunological staining of NPC biopsy. Control panel was stained with secondary antibody. BB7.2 antibody stained for HLA-A02. The other two panels were stained with anti-HLA-A0201/LMP2A and anti-HLA-A0201/EBNA1 mAb. Positive staining was observed to be reddish brown. Scale bars, 25 μm. From: Sim AC, Too CT, Oo MZ, Lai J, Eio MY, Song Z, Srinivasan N, Tan DA, Pang SW, Gan SU, Lee KO, Loh TK, Chen J, Chan SH, MacAry PA. Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies. Sci Rep. 2013 Nov 18;3:3232. |
