| 货号 | AHP730 |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | P*, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of formalin fixed, paraffin processed human pancreas with Rabbit anti Human EndoG at 15 µg/ml. (AHP730) | |
Western blot analysis of whole cell lysates from mouse 3T3 (M) and Human HepG2 (H) cells probed with Rabbit anti endonuclease G (AHP730) | |
Published customer image: Rabbit anti endonuclease G antibody (AHP730) used for the evaluation of Endo G expression in rat mitochondria by western blotting. Image caption: Release of mitochondrial intermembrane space proteins. Mitochondria were incubated as described in the legend of fig. 1, whereupon they were sedimented by centrifugation and proteins of the resulting pellets (P) and supernatants (S) were analyzed by immunoblotting. Panel A: release of cytochrome c, Smac/DIABLO, Omi/HtrA2, AIF, endonuclease G, and LACTB by histones, tBID and p53. The concentrations used were: 5 μM histone H1.2; 10 μM histone H2A, 10 μM histone H2B, 5 μM histone H3, 5 μM histone H4, 1 μM tBID, and 1 μM p53. Panel B: effect of cyclosporin A on the histone-induced release of cytochrome c and Smac/DIABLO. Panel C: accessibility of AIF and LACTB to trypsin hydrolysis in the presence of histones. From: Cascone A, Bruelle C, Lindholm D, Bernardi P, Eriksson O (2012) Destabilization of the Outer and Inner Mitochondrial Membranes by Core and Linker Histones. PLoS ONE 7(4): e35357. | |
Published customer image: Rabbit anti endonuclease G antibody (AHP730) used for the evaluation of EndoG expression in HCC1187 cells treated with TG02 in vitro by western blotting. Image caption: TG02 triggers apoptosis through caspase-dependent and independent pathways (A) HCC1187 cells were treated with TG02 (1 μM) for different times (0, 6, 12 and 24 hours). Loss of mitochondrial membrane potential and viability were analyzed by flow cytometry after staining with TMRE or propidium iodide (PI), respectively. (B) Expression of proteins involved in apoptotic mechanisms was analyzed by Western blot after treatment of HCC1187 cells with TG02 (1 μM) at the indicated times. GAPDH was used as loading control. (C) Release of proteins from the intermembrane space of mitochondria in HCC1187 cells treated for 12 and 24 hours with TG02 (1 μM) was analyzed by Western blot after subcellular fractionation. COX IV and ERK1/2 were analyzed as mitochondrial and cytosolic markers, respectively. (D) Role of caspases in TG02-induced cell death. Induction of cell death by TG02 was analyzed in HCC1187 cells by flow cytometry after 1 hour of pretreatment with the pan-caspase inhibitor Z-VAD-FMK (25 μM), followed by treatment with TG02 (1 μM) for 24 hours. From: Ortiz-Ruiz MJ, Álvarez-Fernández S, Parrott T, Zaknoen S, Burrows FJ, Ocaña A, Pandiella A, Esparís-Ogando A. Therapeutic potential of ERK5 targeting in triple negative breast cancer. Oncotarget. 2014 Nov 30;5(22):11308-18. |
