Cotton Rat CD8 alpha Phycoerythrin MAb (Clone JG12) (100 TESTS)
货号：
FAB7080P 基本售价：
2825.6 元 规格：
100 Tests
产品信息
概述| 货号 | FAB7080P |
| 别名 | CD8; CD8A |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects cotton rat CD8 alpha in direct ELISAs. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 925 (Human); 12525 (Mouse); 24930 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NIH‑3T3 mouse embryonic fibroblast cell line transfected with cotton rat CD8 alpha and SP2/O mouse melanoma cell line transfected with cotton rat CD8 alpha.
Accession # AAL55392 |
| 生物活性 | Cotton Rat |
| 标记 | Phycoerythrin |
| 背景 | CD8 alpha (CD8 alpha ; also OX8, Leu-2 and Lyt-2) is a 23 kDa (predicted) member of the Ig superfamily of molecules. It is found on immature thymocytes, cytotoxic and suppressor T cells, select mast cells, and splenic and bone marrow dendritic cells (in rodent). On T cells, CD8 alpha exists as either a disulfide linked homodimer, or heterodimer bound to CD8 beta. CD8 alpha beta is best known as a coreceptor for the TCR, enhancing TCR signaling. CD8 alpha alpha serves a different function and acts as a TCR corepressor that blocks T cell activation. Based on rat, mature Sigmodon hispidus (cotton rat) CD8 alpha is a 210 amino acid (aa) type I transmembrane protein that consists of a 161 aa extracellular region (aa 24-184) with one V-type Ig-like domain (aa 35-135), and a 30 aa cytoplasmic tail (aa 206-235). Although Sigmodon hispidus is called a rat, it is not. It is a rodent, and rat CD8 alpha is the closest ortholog to cotton rat CD8 alpha currently reported. Over the extracellular region, rat and cotton rat CD8 alpha share only 54% aa identity. Rat CD8 alpha runs at 34-39 kDa in SDS-PAGE, and possesses one utilized N-linked and O-linked glycosylation site (vs. no N-linked sites in cotton rat CD8 alpha ). Based on this organization, and assuming O-linked glycosylation, cotton rat CD8 alpha would be expected to run at about 30-34 kDa in SDS-PAGE. Over aa 24-182, cotton rat CD8 alpha shares 54% and 48% aa identity with rat and mouse CD8 alpha, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Cancer Biomarkers
- Conventional/Classical Dendritic Cells
- gamma delta T Cells
- Human CD Marker Products
- ICC / IHC Images: CD8
- Leukocyte Receptors/Markers in Allergy and Asthma
- Natural Killer T (NKT) Cells
- Regulatory T Cells (Tregs)
- T Cell Antigen Recognition
|
参考图片Detection of CD8 alpha in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD8 alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB7080P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
