MOUSE ANTI PCNA【科瑞奥博】

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MOUSE ANTI PCNA

货号： MCA1558 基本售价： 2545.0 元规格： 0.1 mg

产品信息

概述
货号	MCA1558
克隆号	PC10
同种亚型	IgG2a
反应种属	Human
来源宿主	Mouse
应用	C, F*, IP, P, WB

性能
供应商	Bio-Rad Antibodies
运输条件
存放说明	Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC or at -20^oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (MCA1588)

Staining of KM-H2 cells with Mouse anti PCNA:FITC (MCA1558F) permeabilised with Leucoperm (BUF09) and methanol

K562 erythroleukemic whole cell lysate was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Mouse anti Human PCNA (MCA1558) was used as primary antibody followed by a Goat anti Mouse IgG (H/L):HRP secondary antibody at a dilution of 1/10,000 (STAR207P) was used as the secondary antibody. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale

K562 erythroleukemic whole cell lysate was run in lane 2 under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. MCA1558P Mouse anti Human PCNA:HRP conjugate was used as a direct label at a 1/1000 dilution. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale

Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green