| 货号 | 640005 |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Chemical |
| 应用 | #N/A |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS by ELISA Image caption: Comparison of LPS binding in indirect ELISA with different buffers and blocking agents. FITC-LPS from E. coli serotype O111:B4 was incubated at 1.25 μg/ml in PBS-EDTA (PBS), with or without 0.05% Tween 20 (PBS-TW2) or Triton X-100 (PBS-TTX), in wells coated with polymyxin B (PMX) or lysozyme (LSZ) (1 μg/well), or empty wells (control), which were previously blocked with BSA (A) or OVA (B). Each assay was performed in triplicate. Mean values ± SD are shown in the graph. From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016) Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides. PLoS ONE 11(6): e0156530. | |
Published customer image: HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS in a competitive hCAS ELISA Image caption: Competitive hCAS-ELISA performed under neutral conditions. FITC-LPSs (5 μg/ml) from E. coli serotypes O111:B4 (A) and O55:B5 (B) were preincubated with different concentrations of proteins and peptides (40, 10, 1 and 0.25 μg/ml) in PBS-EDTA (pH 7.2) and added to the wells coated with casein hydrolysate (hCAS). Data are expressed as percentage inhibition of LPS binding to hCAS by the target molecule and are the mean values ±SD of duplicate wells. The average values of the optical density (492 nm) of control wells (without inhibitor) were 0.311 (±0.019, red dashed line) and 0.300 (±0.025, red dashed line) for serotypes O111:B4 and O55:B5, respectively. Differences were considered significant at p <0.05. NS: not significant. BSA, bovine serum albumin; HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, A. simplex peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B. From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016) Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides. PLoS ONE 11(6): e0156530. | |
Published customer image: HRP conjugated Sheep anti FITC antibody (640005) used for the detection of FITC labeled LPS in a competitive PMX ELISA Image caption: Competitive PMX-ELISA. FITC-LPSs from E. coli serotypes O111:B4 (A) and O55:B5 (B) were preincubated, at 1.25 and 2.5 μg/ml, respectively, with three different concentrations of proteins and peptides (10, 1 and 0.25 μg/ml) in PBS-EDTA with 0.1% BSA and added to wells containing biotinylated polymyxin B captured by deglycosylated avidin. Results are expressed as percentage inhibition of LPS binding to captured polymyxin B by the target protein/peptide, and are the mean values ±SD for duplicate wells. The average optical densities (492 nm) of control wells (without inhibitor) were 0.904 (±0.042, red dashed line) and 0.842 (±0.036, red dashed line) for serotypes O111:B4 and O55:B5, respectively. Differences were considered significant at p <0.05. NS: not significant. HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, A. simplex peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B. From: Martínez-Sernández V, Orbegozo-Medina RA, Romarís F, Paniagua E, Ubeira FM (2016) Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides. PLoS ONE 11(6): e0156530. |
