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Sheep antiGreen Fluorescent protein antibody (4745-1051) used to detect GFP tagged constructs (CG8784:p65) in Drosophila larvae by immunofluorescence.
Image caption:
Hugin neurons target neurosecretory cells via peptide-receptor transmission in addition to synaptic connections. A, Promoter-based hugin receptor PK2-R1 driver line CG8784-Gal4::p65 was generated by replacing the first coding exon of the CG8784 loci with GAL4 in a BAC clone containing 80kb flanking genomic context and integrating the final BAC into attP site VK00033. B, CG8784-Gal4::p65 drives expression in cells of the pars intercerebralis (PI). C, Co-staining with Drosophila insulin-like peptide 2 (Dilp2), diuretic hormone 44 (DH44) and Dromyosuppressin (DMS). These peptides are produced by medial neurosecretory cells (mNSCs) in a non-overlapping manner. CG8784-Gal4::p65 drives expression in all mNSCs of the PI. Scale bars in A and B represent 10 &mum.

From: Schlegel P, Texada MJ, Miroschnikow A, Schoofs A, Hückesfeld S, Peters M,Schneider-Mizell CM, Lacin H, Li F, Fetter RD, Truman JW, Cardona A, Pankratz MJ.
Synaptic transmission parallels neuromodulation in a central food-intake circuit.
Elife. 2016 Nov 15;5. pii: e16799.

Published customer image:
Sheep anti green fluorescent protein antibody (4745-1051) used for the detection of GFP tagged protein by immunofluorescence.
Image caption:
Khc is dispensable for early eye pattern formation. (A) A null mutant, khc8, was utilized to generate mutant clones in developing third-instar larval eye discs. Crb (apical marker, red) and E-cad (AJ maker, blue) showed little defects in third-instar larval eye discs. (B) A magnified view of (A). Mutant clones were marked by the absence of GFP (green).

From: League GP, Nam S-C (2011)
Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in DrosophilaPhotoreceptor.
PLoS ONE 6(6): e21218.

Published customer image:
Sheep anti green fluorescent protein antibody (4745-1051)used for the detection of GFP tagged protein by immunofluorescence.
Image caption:
Khc is essential for photoreceptor morphogenesis. khc8 mosaic clone in mid-stage (50% pd) pupal Drosophila photoreceptors. (A–E) khc8 Drosophila photoreceptors stained for Crb (apical marker, red), and E-cad (AJ marker, blue). (A′–E′) khc8 mutant photoreceptors were marked by the absence of GFP (green). As the cross sections move more proximally (A–E) both the apical Crb domain (red) and the AJs (blue) show increasingly severe defects. Crb (red) is misshapen at the distal section (A, B) and almost absent at the proximal section (E) from the same pupal eye. (F) Developing pupal photoreceptors elongate from distal to proximal direction. Distal (A, B) and proximal (D, E) sections are marked by dashed-lines.

From: League GP, Nam S-C (2011) Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in DrosophilaPhotoreceptor.PLoS ONE 6(6): e21218.

Published customer image:
Sheep anti green fluorescent protein antibody (4745-1051) used for the detection of GFP tagged protein by immunofluorescence.
Image caption:
Khc is essential for acetylated microtubules and AJ localization. Stabilized microtubules were stained with Acetub (red), AJs with E-cad (blue), and wild type cells with GFP (green). (A-A′) Distal regions of khc8 mutant photoreceptors, marked by the absence of GFP (green), display reduction and shrinkage of microtubules (arrow) compared to wild-type cells (double-arrow) and AJs (arrowhead) at the same location. (B-B′) Proximal regions of khc8 mutant photoreceptors display more severe defects of acetylated microtubules (arrow) and AJs (arrowhead).

From: League GP, Nam S-C (2011)
Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in DrosophilaPhotoreceptor.
PLoS ONE 6(6): e21218.

Published customer image:
Sheep anti green fluorescent protein antibody (4745-1051) used for the detection of GFP tagged protein by immunofluorescence.
Image caption:
Overexpression of Khc causes severe reduction of acetylated microtubules and apical domain. Khc-GFP overexpression causes severe reduction of the apical membrane domain in developing pupal eyes (45% pd). (A, B) Localization of Crb (apical marker, red) and E-cad (AJ marker, blue) in the sev-Gal4/+ control (A), and Khc-GFP overexpression by sev-Gal4 (B). Khc-GFP was marked by GFP (green, B′). Khc-GFP caused a loss of Crb (red, arrow, B) and mislocalization of E-cad (blue). (C, D) Localization of Acetub (stabilized microtubule marker, red) and Crb (apical marker, blue) in the sev-Gal4/+ control (C), and sev>Khc-GFP (D). Gal4 caused the reduction of Acetub (red, arrow, D) and Crb (blue). Khc-GFP was marked by GFP (green, D′).

From: League GP, Nam S-C (2011)
Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in DrosophilaPhotoreceptor.
PLoS ONE 6(6): e21218.

Published customer image:
Sheep anti green fluorescent protein antibody (4745-1051) used for the detection of GFP by immunofluorescence.
Image caption:
Properties of NG2 cell processes.
(A–D) Two-photon time-lapse recordings. (A) Overview of an Alexa-594-labeled NG2/EYFP cell (maximum projection, 100 μm x 100 μm x 15 μm, 60 equidistant planes, scale 10 μm). (B–D) Pairs of maximum projections (16 μm x 14 μm x 5 μm, 20 planes, scale 2 μm) taken at time points t0 (left) and t0 + Δt (right). Arrows mark processes that were elongated (B, Δt ?=?185 s) or retracted (C, Δt?=?370 s). Additionally we observed varicosities traveling along the process (D, Δt?=?370 s, start and end point marked by arrows). See also Videos S2, S3. (E–H) NG2 cell processes do not contain α-tubulin and PDI. Cortical tissue from an hGFAP/EGFP mouse (p13) was freshly dissociated and quadruple-stained with a nuclear marker (bisbenzimidine, blue) and antibodies against GFAP (also blue channel), GFP (green) and one of the proteins of interest (red): &apha;-tubulin (E), β-actin (F), ezrin (G) or the ER marker PDI (H). The cells analyzed were GFAP negative, GFP positive. Note nearby GFP negative cells (overviews, left in F–H, E). Areas boxed in the overviews (F–H) are enlarged for colocalization analysis. β-actin and ezrin were localized in the NG2 cell processes. Note the fine dimensions of these varicose processes visualized in the GFP channel (green). The PDI signal is present both in a non-identified, nearby cell (H, overview) and in the soma of the NG2 cell, but not in its processes (H, red, merge). The same is observed for α-tubulin (E red, merge). Note that α-tubulin/microtubules are well-preserved in the processes of nearby non-identified cells (E, merge) and of GFAP positive astrocytes (cf. Fig. S1). Scale bar 5 μm.

From: Haberlandt C, Derouiche A, Wyczynski A, Haseleu J, Pohle J, et al. (2011) Gray Matter NG2 Cells Display Multiple Ca2+-Signaling Pathways and Highly Motile Processes. PLoS ONE 6(3): e17575.

Published customer image:
Sheep anti green fluorescent protein antibody (4745-1051) used for the detection of GFP tagged Hb9 neurons by immunofluorescence.
Image caption:
Gfrα1-TLZ+/Hb9::GFP- motor neurons display structural and synaptic characteristics of gamma motor neurons. (A) Neurolucida tracings of P20 large Gfrα1-TLZ-/Hb9::GFP+ (black) and small strongly Gfrα1-TLZ+/Hb9::GFP- MNs (gray). (B-D) Quantitative analyses of dendritic arbors. Gfr&apha;1-TLZ-/Hb9::GFP+ MN primary dendrites are more numerous, more highly branched (B) and thicker (C), than those of Gfrα1+/Hb9::GFP- MNs. Sholl analysis (D) of Gfrα1-TLZ-/Hb9::GFP+ (black line) and Gfrα1-TLZ+/Hb9::GFP- (gray line) MNs also reveals differences in the distribution of membrane surface at different distances from soma that are characteristic of α- vs. γ-MNs. (E) VGluT1+ contacts (red) are present on P20 Hb9::GFP+ (green) MNs, but absent on Gfrα1-TLZ+ (blue)/Hb9::GFP- neurons. (F) VAChT+ contacts (red) are present on both Hb9::GFP+ (green) and Gfrα1-TLZ+ (blue)/Hb9::GFP- MNs (E and F, regions in white boxes are magnified in insets). (G) Quantification of dendritic and somatic VGluT1 and VAChT positive contacts on Hb9::GFP+ (black bars) and Gfrα1-TLZ+/Hb9::GFP- (gray bars) MNs (error bars indicate SEMs; triple and double asterisks indicate significance levels of P<0.001 and P<0.01 in t-test comparisons, respectively). (H-K) Tibialis anterior muscle in a Hb9::GFP mouse showing Cy5-bungarotoxin (Cy5-Bgtx, blue) labeled intra- and extrafusal neuromuscular junctions (NMJ) and PGP9.5 immunolabeled sensory and motor axons (red). Hb9::GFP+ motor axons are in green (H). Spindle afferent annulospiral endings (dual asterisks; also shown in the inset in a serial section) and intrafusal muscle fibers are labeled with PGP9.5. Extrafusal NMJs are innervated by PGP9.5+ and Hb9::GFP+ motor axons (K, high magnification of boxed area). Most motor end-plates on intrafusal fibers lacked GFP. Intrafusal Cy5-Bgtx NMJs (white arrowheads) are innervated by PGP9.5-IR axons that are HB9::GFP- (I, high magnification of boxed area). Yellow arrowheads indicate a few intrafusal NMJs innervated by PGP9.5+ and Hb9::GFP+ motor axons (example boxed and shown at higher magnification in J). Scale bars: (E,F) 20 μm; (H) 200 μm (100 μm in inset); (I,J) 25 μm.

From: Shneider NA, Brown MN, Smith CA, Pickel J, Alvarez FJ.
Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival.
Neural Dev. 2009 Dec 2;4:42.