DYKDDDDK Epitope Tag Alexa Fluor 488 MAb (Clone 1042E) (25 TESTS)
货号：
IC8529G-025 基本售价：
1824.2 元 规格：
25 Tests
产品信息
概述| 货号 | IC8529G-025 |
| 简介 | Recombinant Monoclonal Antibody |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | DYKDDDDK epitope tag |
| 标记 | Alexa Fluor 488 |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
参考图片Detection of DYKDDDDK (FLAG®) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor® 488‑conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529G, filled histogram) or isotype control antibody (Catalog # IC105G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
