c-myc tagged protein detected with Mouse anti c-myc:Alk Phos (MCA2200A)

Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody (MCA2200) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence

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Alexa-FluorA647 conjugated Mouse anti Human c-myc antibody, clone 9E10 (MCA2200A647) used for the evaluation of c-myc on dicidual NK cells demonstrating the correlation between c-myc and CD122 expression.
Image caption:
Expansion of specific iNKR subsets in decidual NK cells compared with matched pbNK. The expression of KIR2DL1, KIR2DL3/L2/S2, KIR3DL1, LILRB1, and NKG2A was examined by nine-color flow cytometry in matched pbNK and dNK from 21 donors. (A) Thirty-two distinct NK subsets were distinguished on the basis of the five iNKR in paired samples of pbNK and dNK (black and red graphs, respectively). The frequency of each subset from one representative donor is shown as a percentage of total NK cells. This provides a “fingerprint” of the iNKR repertoire, which is characteristic for each donor. The receptor combination for each subset is denoted by black-filled circles, with total number of iNKR in each subset indicated beneath. All NKG2A+ subsets are grouped to the right. The donor shown was genotyped as C1/C1. (B) The proportion of pbNK and dNK that express specific receptor combinations is shown as a percentage of total NK cells (mean ± SD, n = 21 matched pbNK and dNK pairs). The proportion of dNK that express two or more iNKR is significantly higher than in pbNK. (C) The percentage of dNK cells that are positive for Ki-67 was determined in each iNKR subset by intracellular staining. Control staining used isotype-matched mAb. The NK cells shown were gated on total KIR2DL1+ dNK as shown in Fig. 1A. (D) The percentages of dNK that are positive by intracellular staining for Ki-67 (•) and for CD122 (▴) are shown for all NKG2A+ dNK subsets (mean for each subset indicated by • or ▴ ± SD, derived from n = 11 donors). (E) Mean fluorescence intensity (MFI) of staining of each subset is shown following staining for CD122 and c-Myc in the NKG2A+ dNK subsets from (D). For each donor, MFI of each subset was expressed relative to the subset expressing NKG2A alone, which was set at 100%. Relative MFI of CD122 and c-Myc is highly correlated (Spearman rank correlation coefficient, R2 = 0.84, p <0.001).

From: Sharkey AM, Xiong S, Kennedy PR, Gardner L, Farrell LE, Chazara O, Ivarsson MA, Hiby SE, Colucci F, Moffett A.
Tissue-Specific Education of Decidual NK Cells.
J Immunol. 2015 Oct 1;195(7):3026-32.