| 货号 | 6235S |
| 描述 | SignalSilence® Jak2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Jak2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. |
| 存放说明 | -20C |
| 参考文献 | Leonard, W.J. and OShea, J.J. (1998) Annu Rev Immunol 16, 293-322. Darnell, J.E. (1997) Science 277, 1630-5. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9. |
Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Jak2 siRNA I (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用Jak2 (D2E12) XP® Rabbit mAb #3230 或α-Tubulin (11H10) Rabbit mAb #2125 研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® Jak2 siRNA I (+) 的K-562 细胞的细胞提取液。The Jak2 (D2E12) XP® Rabbit mAb 证实了对Jak2 蛋白表达的沉默, α-Tubulin (11H10) Rabbit mAb作为上样量的对照。 |
