| 货号 | 7747S |
| 描述 | SignalSilence® USP10 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit USP10 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP10 possesses amino acid sequences that match the consensus cysteine and histidine boxes representative of the USP family of deubiquitinating enzymes. At the posttranslational level, USP10 appears to be regulated through both protein-protein interactions and phosphorylation. Indeed, interaction of USP10 with Ras-GAP SH3 domain binding protein (G3BP) has been found to inhibit its ability to catalyze the disassembly of ubiquitin chains (3). Furthermore, ATM-mediated phosphorylation of USP10 at Thr42 and Ser337 was shown to promote USP10 stabilization and redistribution from the cytoplasm to the nucleus, where it functions in p53 deubiquitination, stabilization, and activation in response to genotoxic stress (4). Recently, it was shown that USP10 works in concert with USP13 and Vps34 complexes. USP10, along with USP13, appears to deubiquitinate Vps34 complexes to regulate the levels of this class III PI3K. Beclin-1, another component of these complexes, functions to regulate the stability of USP13, which can deubiquitinate and stabilize the levels of USP10. Therefore, Beclin-1, can indirectly regulate p53 stability by controlling the DUB activity of USP10 (5). USP10 also functions in the endosomal compartment, where it has been shown to deubiquitinate CFTR in order to enhance its endocytic recycling and cell surface expression (6,7). |
| 存放说明 | -20C |
| 参考文献 | Nijman, S.M. et al. (2005) Cell 123, 773-86. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. Soncini, C. et al. (2001) Oncogene 20, 3869-79. Yuan, J. et al. (2010) Cell 140, 384-96. Liu, J. et al. (2011) Cell 147, 223-34. Bomberger, J.M. et al. (2009) J Biol Chem 284, 18778-89. Bomberger, J.M. et al. (2011) Channels (Austin) 4, 150-4. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® USP10 siRNA I (+), using USP10 (D7A5) Rabbit mAb #8501 and GAPDH (14C10) Rabbit mAb #2118. The USP10 (D7A5) Rabbit mAb confirms silencing of USP10 expression, while the GAPDH (14C10) Rabbit mAb is used as a loading control. 对转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或者 SignalSilence® USP10 siRNA I (+)的293T细胞使用USP10 (D7A5) Rabbit mAb兔单抗 #8501和GAPDH (14C10) Rabbit mAb兔单抗 #2118进行Western blot分析。USP10 (D7A5) Rabbit mAb兔单抗证实了USP10的表达沉默，GAPDH (14C10) Rabbit mAb兔单抗用作上样对照。 |
