| 货号 | 61489 |
| 别名 | ORFX, RING3L |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq & ChIP-chip: 10 µl each WB: 1:500- 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the C-terminal region of mouse BRD3. |
| 背景 | BRD3 (Bromodomain Containing 3) is a member of the mammalian BET (Bromodomain Extra Terminal) family of proteins, binds hyperacetylated chromatin and has been reported to interact with acetylated Histone H4K5, Histone H4K12 and Histone H3K14. It plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 100 kDa |
| 参考文献 |
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BRD3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human H526 cells and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to show the expected promoter localization. | |
BRD3 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human H526 cells and 10 µl BRD3 antibody. ChIP DNA was used in qPCR with the control primer or gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
BRD3 antibody (pAb) tested by Western blot. Detection of BRD3 by Western blot analysis. Lane 1: Whole cell extract of HeLa cells (20 µg). Lane 2: Whole cell extract of NIH-3T3 cells. Both probed with BRD3 antibody (pAb) at a 1:500 dilution. |
